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Recombinant Rat IL-6 (carrier-free)
Recombinant Rat IL-6 (carrier-free)
579902
10 µg
¥25,000
579904
25 µg
¥45,000
579906
100 µg
¥110,000
579908
500 µg
¥320,000
Product Details
Source:
Rat IL-6, amino acids Phe25-Thr211 (Accession # NM_012589), was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in 38% Acetonitrile, 0.1% TFA.
Endotoxin Level:
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Preparation:
10-100µg sizes are bottled at 200µg/mL. 500µg sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. Do not freeze. Freezing has been shown to reduce bioactivity.
Activity:
ED50 is < 0.01 ng/ml, corresponding to a specific activity of > 1 x 108 units/mg, as determined by the dose dependent stimulation of 7TD1 cell proliferation.
The cDNA sequence coding for the mature portion of rat IL-6 is 92% identical with the corresponding murine sequence and 68% identical with the corresponding human sequence. The two rodent IL6 species are highly conserved. IL-6 is a multifunctional cytokine with both differentiation and growth-promoting effects for a variety of target cell types. It is a B-cell stimulatory factor inducing terminal differentiation and high level antibody production in B lymphocytes (IL6/BSF-2) and it acts as a hybridoma/plasmacytoma growth factor. IL-6 stimulates the growth of hemopoietic stem cells, and induces the synthesis of acute phase plasma proteins (2). In addition, IL-6 and tumor necrosis factor alpha (TNF-alpha), involved into osteoclastogenesis and responsible for bone resorption process, participate in the pathogenesis of the osteoporosis (3). IL-6 plays a pivotal role in liver regeneration (4). It is systemically elevated in obesity and is a predictive factor to develop type 2 diabetes, and regulates pancreatic alpha cell mass expansion (5).
IL-6 is released by activated T cells, B cells, monocytes, macrophages, fibroblasts, keratinocytes, epithelial, endothelial, and synovial cells. In central nervous system, microglia and astrocytes are the main source of IL-6 (1)
Function:
Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Ligand Receptor:
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130) and soluble IL-6R
Interaction:
T cells, B cells, hepatocytes, cholinergic neurons
Antigen References:
1. Quian J, et al.J Am J Physiol Cell Physiol 293:C897-C905 2007. 2. Northemann W, et al.J Bio Chem 264:16072-16082 1989. 3. Zhu X, et al.Transl Res 152:18-23 2008. 4. Pachówka M, et al.Ann Transplant 13:15-19 2008. 5. Ellingsgaard H, et al.P. Natl. Acad. Sci. USA 105:13163-13168 2008.
Recombinant Rat IL-6 (carrier-free), Recombinant, Interleukin-6, Interferon-β2, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF), Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
