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Recombinant Mouse VEGF120 (carrier-free)
Recombinant Mouse VEGF120 (carrier-free)
580902
10 µg
¥39,000
580904
25 µg
¥65,000
580906
100 µg
¥160,000
580908
500 µg
¥360,000
Product Details
Source:
Mouse VEGF120, amino acids Ala27-Arg146 (Accession # S38100) was expressed in E. coli.
Formulation:
0.22µm filtered protein solution is in 5mM Citric Acid, 5mM NaH2PO4, 150mM NaCl, pH 4.0.
Endotoxin Level:
Endotoxin level is <0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method.
Preparation:
10-100µg sizes are bottled at 200µg/mL. 500µg sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at -20°C for six months or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity:
ED50 = 1-4 ng/ml, corresponding to a specific activity of 1-0.25 x 106 units/mg, as determined by the dose dependent stimulation of HUVEC cells proliferation.
VegfA belong to the VEGF family which has at least 7 members, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, PlGF and snake venom-derived VEGFs such as T.f. (Trimeresurus flavoviridis) svVEGF. In humans, VEGF-A is highly expressed in most of the solid tumors generated in breast, lung, renal, colorectal and liver tissues. VEGF-A has strong vascular permeability activity, and significantly contributes to the formation of ascites tumors. VEGF can act as a direct proinflammatory mediator during the pathogenesis of RA, and protect rheumatoid synoviocytes from apoptosis, which contributes to synovial hyperplasia. VEGF is expressed in synovial macrophages and synovial fibroblasts in the synovial tissues of RA patients.
Other Names:
Vegf, Vegf-a, Vegf120, Vpf
Structure:
cytokine
Distribution:
Widely expressed
Function:
VegfA is a key player in vasculogenesis, the formation of blood vessels from progenitor cells, as well as angiogenesis. The expression of the Vegf-a gene is upregulated via hypoxia, estrogen, and NF-κB pathways. VegfA induces proliferation and cell migration in endothelial cells, and plays important roles during wound healing. In addition, VEGF regulates haematopoietic stem cell survival.
Ligand Receptor:
Vascular endothelial growth factor (VEGF)-A binds and activates two tyrosine kinase receptors, VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1).
Interaction:
VegfA interacts with vascular endothelial and monocytes/macrophages, which express VEGFR1. This interaction induces proliferation of endothelial cells, and stimulates migration of monocytes/macrophages. VEGFR2 is expressed in endothelial cells and VEGFR2-
Antigen References:
1. Shibuya M, et al.J Biochem Mol Biol 39:469-478 2006. 2. Shibuya M, et al.BMB Rep. 2008 41(4):278-86 2008. 3. Yoo SA, et al.Hindawi Publishing Corporation Mediators of Inflammation Volume 2008 Article ID 129873 4. Conn G, et al.Proc. Nati. Acad. Sci. 87:1323-1327 1990. 5. Gerber H, et al.Nature 417:954-958 2002.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
