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Recombinant Mouse TNF-α (carrier-free)
Recombinant Mouse TNF-α (carrier-free)
575202
10 µg
¥25,000
575204
25 µg
¥45,000
575206
100 µg
¥110,000
575208
500 µg
¥295,000
Product Details
Source:
Mouse TNF-α, amino acids Leu80-Leu235 (Accession # NM_013693), was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
Endotoxin Level:
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Preparation:
10-100µg sizes are bottled at 200µg/ml. 500µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity:
The ED50 is 0.010-0.020 ng/ml, corresponding to a specific activity of 5-10 X107 units/mg, as determined by a dose dependent stimulation of L929 cells treated with actinomycin D.
Use when high specific biological activity is required.
COA:
Description:
TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,
Cellular Sources:
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
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