Mouse TACI (Accession# NM_021349: Met1-Thr 129) - SRENLYFQG - Human IgG (Pro 100 - Lys300) was expressed 293E cells.
The total predicted molecular weight is 41 kD. The non-reduced protein migrates between 80-90 kD and the DTT-reduced at approximately 45 - 50 kD by SDS-PAGE.
>98%, as determined by Coomassie stained SDS-PAGE.
0.22 µm filtered protein solution is in PBS
Less than 0.01 ng per µg cytokine as determined by the LAL method.
10-100 µg sizes are bottled at 200 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
10 - 500 µg size are bottled at 0.200 mg/mL.
Storage & Handling:
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20°C to -70°C. Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
ED50 =5 - 10 ng/ml, corresponding to a specific activity of 1.0-2.0 x 105 units/mg, as determined by inhibition of BAFF mediated B cell proliferation by mouse TACI-Fc in a dose dependent manner. B cells were activated with anti-IgM
Inhibition of BAFF mediated B cell proliferation by mouse TACI-Fc.
Transmembrane activator and calcium-modulator and cytophilin ligand interactor (TACI) is a TNFR family member molecule and is part of the so-called BAFF system. This includes two ligands: B cell-activating factor of the TNF family (BAFF) and a proliferation-inducing ligand (APRIL), and three receptors: BAFFR (TNFRSF13C), TACI (TNFRSF13B), and B cell maturation Ag (BCMA or TNFRSF17). BAFF binds the three receptors, while APRIL binds TACI and BCMA. TACI is constitutively expressed on naïve B cells and is upregulated on activated B cells. TACI levels are severely reduced in newborn mice B cells compared with those of adult mice, and newborn B cells do not secret Igs when they are stimulated with BAFF or APRIL. This reduced TACI expression might be responsible for the irresponsiveness of newborn B cells to T cell independent type II antigens such as bacterial capsular polysaccharides.
Disulfide linked homodimer
Peripheral B lymphocytes
TACI triggers immunoglobulin class switching, IgG and IgA, by activating B cells through the adaptor MyD88. It also enhances the generation and maintenance of isotype-switched memory B lymphocytes and plasma cells, and increases the affinity of the produced antibody by the process of somatic hypermutation of the Ig gene. TACI drives plasma cell differentiation in LPS activated B cells. In addition, TACI is required in T-independent antibody production and in the regulation of B cell numbers. The interaction between B cells (expressing TACI) and DC (expressing BAFF) induces a rapid DC maturation which primes naive CD8+ T cells.
APRIL (TNFSFL13A, CD256) and BAFF (TNFSFL13B, CD257) are TACI ligands.
B cells, myeloid cells, and monocytes derived dendritic cells.
1. Schneider P, et al. 1999. J. Exp. Med. 189:1747. 2. Diaz-de-Durana Y, et al. 2006. Blood 107:594. 3. Kanswal S, et al. 2008. J. Immunol. 181:976. 4. Yang J, et al. 2009. PLOS One 4:e8494 5. Ozcan E, et al. 2009. J. Allergy Clin. Immunol. 123:1277. 6. He B, et al. 2010. Nat. Immunol. 1:836.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.