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Mouse TACI (Accession# NM_021349: Met1-Thr 129) - SRENLYFQG - Human IgG (Pro 100 - Lys300) was expressed 293E cells.
The total predicted molecular weight is 41 kD. The non-reduced protein migrates between 80-90 kD and the DTT-reduced at approximately 45 - 50 kD by SDS-PAGE.
>98%, as determined by Coomassie stained SDS-PAGE.
0.22 µm filtered protein solution is in PBS
Less than 0.01 ng per µg cytokine as determined by the LAL method.
10-100 µg sizes are bottled at 200 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
10 - 500 µg size are bottled at 0.200 mg/mL.
Storage & Handling:
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20°C to -70°C. Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
ED50 =5 - 10 ng/ml, corresponding to a specific activity of 1.0-2.0 x 105 units/mg, as determined by inhibition of BAFF mediated B cell proliferation by mouse TACI-Fc in a dose dependent manner. B cells were activated with anti-IgM
Inhibition of BAFF mediated B cell proliferation by mouse TACI-Fc.
Transmembrane activator and calcium-modulator and cytophilin ligand interactor (TACI) is a TNFR family member molecule and is part of the so-called BAFF system. This includes two ligands: B cell-activating factor of the TNF family (BAFF) and a proliferation-inducing ligand (APRIL), and three receptors: BAFFR (TNFRSF13C), TACI (TNFRSF13B), and B cell maturation Ag (BCMA or TNFRSF17). BAFF binds the three receptors, while APRIL binds TACI and BCMA. TACI is constitutively expressed on naïve B cells and is upregulated on activated B cells. TACI levels are severely reduced in newborn mice B cells compared with those of adult mice, and newborn B cells do not secret Igs when they are stimulated with BAFF or APRIL. This reduced TACI expression might be responsible for the irresponsiveness of newborn B cells to T cell independent type II antigens such as bacterial capsular polysaccharides.
Disulfide linked homodimer
Peripheral B lymphocytes
TACI triggers immunoglobulin class switching, IgG and IgA, by activating B cells through the adaptor MyD88. It also enhances the generation and maintenance of isotype-switched memory B lymphocytes and plasma cells, and increases the affinity of the produced antibody by the process of somatic hypermutation of the Ig gene. TACI drives plasma cell differentiation in LPS activated B cells. In addition, TACI is required in T-independent antibody production and in the regulation of B cell numbers. The interaction between B cells (expressing TACI) and DC (expressing BAFF) induces a rapid DC maturation which primes naive CD8+ T cells.
APRIL (TNFSFL13A, CD256) and BAFF (TNFSFL13B, CD257) are TACI ligands.
B cells, myeloid cells, and monocytes derived dendritic cells.
1. Schneider P, et al. 1999. J. Exp. Med. 189:1747. 2. Diaz-de-Durana Y, et al. 2006. Blood 107:594. 3. Kanswal S, et al. 2008. J. Immunol. 181:976. 4. Yang J, et al. 2009. PLOS One 4:e8494 5. Ozcan E, et al. 2009. J. Allergy Clin. Immunol. 123:1277. 6. He B, et al. 2010. Nat. Immunol. 1:836.
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