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Recombinant Mouse SCF (carrier-free)
Recombinant Mouse SCF (carrier-free)
579702
10 µg
¥35,000
579704
25 µg
¥65,000
579706
100 µg
¥145,000
579708
500 µg
¥295,000
Product Details
Source:
Mouse SCF, amino acids Lys26-Ala189 (Accession# M59915) was expressed in E. coli.
Formulation:
0.22µm filtered protein solution is in 10mM NaHPO4 pH 7.2, 0.15M NaCl.
Endotoxin Level:
Less than 0.01ng per µg cytokine as determined by the LAL method.
Concentration:
10-100µg sizes are bottled at 200µg/mL, 500µg sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term-storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity:
ED50 = 15 ng/ml, corresponding to a specific activity of 6.7 x 104 units/mg, as determined by the dose dependent stimulation of TF-1 cell proliferation.
SCF (KITL) is a hematopoietic growth factor, and it can synergize with a number of other cytokines to stimulate growth of hemopoietic progenitors in vitro and stimulates blood cell production in vivo. SCF is encoded by Sl ('steel'), a gene critical to the development of several distinct cell lineages during embryonic life. KITL was identified as a soluble protein; nevertheless, the predicted amino acid sequence indicates that it is an integral transmembrane protein. KITL is generated by proteolytic cleavage from a transmembrane precursor. Two splice variants have been descrived for KITL, and proteolytic processing of both transmembrane protein products occurs on the cell surface. The binding of KITL, induces the dimerization of the KIT molecule, followed by a change in the configuration of the intracellular domain and the autophosphorylation of the receptor, opening several docking sites for signal transduction molecules. Dysregulation of SCF–KI signaling and gain-of-function KIT mutations contribute to the genesis of many cancers, like acute myeloid leukemia, gastrointestinal stromal tumors, and mastocytosis.
SCF has pleiotropic functions. It is involved in melanogenesis, gametogenesis, hematopoiesis and brain angiogenesis.
Ligand Receptor:
SCF binds to the extracellular domains of the KIT receptor tyrosine kinase.
Interaction:
The KIT receptor tyrosine kinase (RTK) is expressed in hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukem
Antigen References:
1. Sun L, et al. 2006 Cancer Cell 9:287-300. 2. Magnol, et al. 2007 BMC Dev Biol. 7:81. 3. Liu H, et al. 2007 EMBO J 26:891-901. 4. Agosti V, et al. 2004 J. Exp. Med. 199:867-878. 5. Huang L el al.1992 Mol Cell Biol 3:349-362. 6. Kitamura T, et al. 1989 J. Cell Physiol. 140:323-334.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
