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Mouse IL-7, amino acids Glu26 -Ile154 (Accession# NM_008371.4) was expressed in insect cells.
Molecular Mass:
The 148 amino acid recombinant protein (with ADL-His9-SSGIEGR at N-terminus) has a predicted molecular mass of approximately 17 kD. The DTT-reduced and non-reduced protein migrates at approximately 20 kD by SDS-PAGE.
Purity:
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation:
The protein was 0.22 µm filtered in 10 mM NaH2PO4, 150 mM NaCl, pH 7.2.
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration:
10 µg and 25 µg sizes are bottled at 100 µg/mL.
Storage & Handling:
Unopened vial can be stored at 4°C for three months or from -20°C to -70°C for six months. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
ED50 =1.0 - 5 ng/ml, corresponding to a specific activity of 0.2 - 1 x 106 units/mg, as determined by PHA activated PBL proliferation induced by IL-7 in a dose dependent manner. Under this condition the bioactivity is equivalent
PHA activated PBL proliferation induced by mouse IL-7.
Description:
Mouse IL-7 was initially described as a pre B-cell grow factor expressed in bone marrow stromal cells. IL-7 is essential for normal murine B cell development, and plays a key role in regulating the homeostasis and function of the T-cells. The binding of IL-7 to its receptor induces dimerization of IL-7Ra and the common gamma chain (gc) which leads to activation of receptor associated tyrosine Janus kinases JAK1 (IL-7R) and JAK3 (γc). The activated JAK proteins in turn phosphorylate specific residues on the IL-7R creating docking sites for signaling molecules such as STAT5 and to a lesser extent STAT1 and STAT3. In adult mice, IL-7/IL-7R signaling up regulates expression of early B cell factor (EBF), and EBF in turn regulates expression of B cell-specific genes required for the transition from lymphoid progenitor to pro-B cells. Besides, IL-7 plays a role in the development of secondary lymphoid tissues. IL-7 is necessary to specify CD8 lineage differentiation during CD4/CD8 cell fate choice in the thymus by inducing expression of the transcription factor Runx3. IL-7 induces anti-apoptotic factors Bcl2 and Bcl-xL and inhibiting pro-apoptotic factors such as Bad and Bax. In this fashion, IL-7 induces cell activation, survival, and proliferation of T lymphocytes. In addition, IL-7 controls T-cell size and metabolism through the activation of PI3 kinase-dependent pathways and regulation of glucose metabolism. IL-7 also controls T cell–dendritic cell interactions that are essential for both T-cell homeostasis and activation in vivo. CD4 T cell lymphopenia increases the expression of circulating IL-7, and TGFb induces IL-7 downregulation.
Thymic and bone morrow stromal cells, keratinocytes, dendritic cells, endothelial cells, intestinal epithelial, and epithelial goblet cells.
Function:
IL-7 is essential for B cell production.
Ligand Receptor:
Heterodimer IL-7Rα (CD127); γ-subunit (CD132) which shares component with IL-2, IL-4, IL-13, IL-15, and IL-21 receptors.
Interaction:
Progenitor B and T cells, CD4-CD8- thymocytes, naïve and memory CD4 and CD8 T cells, and megakaryocytes.
Antigen References:
1. Yokota T, et al. 1986. P. Natl. Acad. Sci. USA 83:5894. 2. Meier D, et al. 2007. Immunity 26:643. 3. Parish JK, et al. 2009 J. Immunol. 182:4255. 4. Saini M, et al. 2009. Blood 113:5793. 5. Park JH, et al. 2010. Nat. Immunol. 11:257. 6. Li WQ, et al. 2010. Mol. Cell. Biol. 30:590.
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