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Recombinant Mouse IL-5
Recombinant Mouse IL-5
563303
5 µg
¥42,000
Product Details
Source:
E. coli, approximately 30-40 kD
Formulation:
Sterile-filtered through a 0.2 micron filter. Lyophilized from 10 mM sodium phosphate, pH 7.5.
Preparation:
Centrifuge vial prior to opening. Reconstitute in 100 µl sterile water for a concentration of 50 µg/ml and mix well. This solution can be diluted into other aqueous buffers that containing a carrier protein such as 1% BSA or HSA or 10% FBS. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer with carrier protein. After reconstitution, the cytokine can be stored at -20°C to -70°C for up to 3 months.
Storage & Handling:
The lyophilized protein is stable for 12 months from the date of receipt at -20°C to -70°C. Reconstituted protein is stable for 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeat freeze/thaw cycles.
Activity:
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.
Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA: This IL-5 protein is useful as a standard for a mouse IL-5 sandwich ELISA, using unlabeled TRFK5 antibody (Cat. No. 504302) for capture and biotinylated TRFK4 antibody (Cat. No. 504402) for detection. Ligand Blocking: This IL-5 protein is useful as a ligand-blocking specificity control for immunohistochemical or immunofluorescent staining. Bioassay: This IL-5 protein is biologically active, and can be used for in vitro assays.
Description:
IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. The native protein can have variable molecular weight as a result of glycosylation. Monomeric mouse IL-5 is a 113 amino acid protein with a reported molecular weight of 35-37 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors and stimulates the activation, and differentiation of eosinophils, and promotes the generation of cytotoxic lymphocytes . Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin.
Other Names:
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)
Structure:
Cytokine; dimer
Cellular Sources:
Mast cells, T cells, eosinophils
Cellular Targets:
Eosinophils, B cells
Receptors:
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Bioactivity/Activities:
Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Takatsu K, et al. 1988. Immunol. Rev. 102:107. 3. Takatsu K. 1992. Curr. Opin. Immunol. 4:299. 4. Takatsu K. 1991. Microbiol. Immun. 35:593.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
