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Recombinant Mouse IL-4 (carrier-free)
Recombinant Mouse IL-4 (carrier-free)
574302
10 µg
¥37,000
574304
25 µg
¥69,000
574306
100 µg
¥175,000
574308
500 µg
¥400,000
Product Details
Source:
Mouse IL-4, amino acids His23-Ser140 (Accession# NM_021283), was expressed in 293E cells using human IL-2 signal peptide.
Formulation:
0.22 µm filtered protein solution is in PBS.
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
10-100 µg sizes are bottled at 200 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or -20°C to -70°C for up to three months. Avoid repeated freeze/thaw cycles.
Activity:
ED50 =2 - 4 ng/ml, corresponding to a specific activity of 2.5 - 5 x 105 units/mg, as determined by CTLL-2 cell proliferation induced by mouse IL-4 in a dose dependent manner.
IL-4 is a member of the short four-helix bundle family of cytokines. IL-4 is produced by Th2 cells in response to antigen receptor engagement. Dendritic cells can prime naïve CD4+ T cell; nevertheless, it has been described that DC-mediated priming is insufficient for the development of Th2 cell-dependent immunity. Basophils have been identified as a source or IL-4 in the initial naïve CD4+ T cell priming. These cells coexpress MHC class II and IL-4 message following helminth infection. Therefore, murine basophils could present antigens to naïve T cells, thus promoting Th2 cell differentiation during allergic and anti-parasitic immune responses. In addition, it was previously known that upon cross-linkage of the high affinity receptor for immunoglobulin E, IL-4 is produced by NK cells, mast cells, and basophils. Cells of hematopoietic stem cell origin express the type I receptor for IL-4 (IL-4Ra,gc), and cells of non-hematopoietic stem cell origin express type II receptor (IL-4Ra, IL-13Ra1). Type I receptor complexes are active in regulating Th2 development. In contrast, the type II receptor complex is active in regulating cells that mediate airway hypersensitivity and mucus secretion.
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