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Recombinant Mouse IL-34 (carrier free)
Recombinant Mouse IL-34 (carrier free)
577602
10 µg
¥55,000
577604
25 µg
¥119,000
577606
100 µg
¥300,000
577608
500 µg
¥840,000
Product Details
Source:
The mouse IL-34 (Accession# NM_001135100.1: Met1- Pro235 with a C-terminal 8His tag) was expressed CHO cells.
Formulation:
0.22 µm filtered protein solution is in 10 mM NaH2PO4, 0.5 M NaCl, pH 7.4
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
10-100 µg sizes are bottled at 100 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or from from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
ED50 =20 - 30 ng/ml, corresponding to a specific activity of 3.3 - 5 x 104 units/mg, as determined by M-NFS-60 cell proliferation induction in a dose dependent manner.
M-NSF-60 cell proliferation induced by mouse IL-34.
Description:
Mouse IL-34 shares a sequence identity of 71% with human IL-34 on the amino acid level, and the IL-34 gene is syntenic in the human, chimpanzee, rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif and does not share sequence homology with M-CSF; nevertheless, it binds to the CSFR. These two cytokines are not identical in biological activity and signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines have different ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests a differential domain binding in the receptor to IL-34 and CSF. As a result, different bioactivities and signal activation kinetics/strength are produced for these cytokines.
Other Names:
C16orf77 homolog
Structure:
Homodimer
Distribution:
IL-34 mRNA is expressed in different tissues, including spleen, heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostate, and colon.
Function:
IL-34 increases monocytes viability, induces macrophage proliferation, and synergized with other cytokines to generate macrophages and osteoclasts from cultured progenitors. IL-34 promotes the formation of the colony-forming unit-macrophage (CFU-M) in human bone marrow culture. IL-34 supports RANKL-induced osteoclastogenesis by promoting the adhesion and proliferation of osteoclast progenitors. IL-1β and TNF-α induce the expression of interleukin-34 mRNA in osteoblasts. IL-34 is induced in macrophages infected with equine infectious anemia virus (EIAV).
Ligand Receptor:
CSF-1R
Interaction:
Macrophages, monocytes, and myeloid cells
Antigen References:
1. Lin H, et al. 2008. Sciences 320:807. 2. Chihara T, et al. 2010. Cell Death Differ. 17:1917. 3. Wei S, et al. 2010. J. Leukoc. Biol. 88:495. 4. Eda H, et al. 2010. Reumathology PMID:21181166. 5. Covaleda L, et al. 2010. Virology 397:217.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
