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Recombinant Mouse IL-33 (carrier-free)
Recombinant Mouse IL-33 (carrier-free)
580502
10 µg
$175.00
580504
25 µg
$325.00
580506
100 µg
$800.00
580508
500 µg
$2,200.00
Product Details
Source:
Mouse IL-33, amino acids Ser109-Ile266 (Accession # NM_133775) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in 20mM HEPES, 150mM NaCl, pH 7.2, 10mM TCEP
Endotoxin Level:
Endotoxin level is < 0.1 EU/µg (< 0.01ng/µg) protein as determined by the LAL method.
Preparation:
10 - 100 µg sizes are bottled at 200 µg/mL. 500 µg sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months or -20°C to -70°C for six months. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term-storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
1. Miller AM, et al. 2010. Circ Res. 107:650. PubMed 2. Zaiss MM, et al. 2011. J. Immunol. 186:6097. PubMed.
IL-5 induction by mouse IL-33 in splenocytes activated by anti-CD3 and anti-CD28. Data kindly provided Dr. Foo Y. Liew.
Description:
IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1b, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-κB and MAPK pathways via identical signalling events to those observed for IL-1b. In addition, IL-33 is a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties.
Other Names:
Il-1f11, Nuclear factor from high endothelial venules (NF-HEV)
Distribution:
High levels of mouse IL-33 mRNA are detected in the stomach, lung, spinal cord, brain, and skin. Expression within these organs is restricted to a few cell types, such as epithelial cells, fibroblasts, and smooth muscle cells. In addition, mouse IL-33 mRNA is found in resting dendritic cells and activated macrophages. Endothelial cells constitute a major source of IL-33 mRNA in chronically inflamed tissues.
Function:
IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. In addition, IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-a and IL-1b are activators of IL-33 transcription in fibroblasts and keratinocytes.
Ligand Receptor:
IL-33 binds to the IL-1 family receptor T1/ST2 and IL-1RAcP (IL-1 receptor associated protein)
Bioactivity/Activities:
Activity was tested by induction of IL-5 in activated splenocytes by IL-33. The ED50 is 2 to 5 ng/ml, corresponding to a specific activity of 5 to 2 x 105 units/mg. Under this assay the activity was comparable to other vendor’s cytokine.
Antigen References:
1. Schmitz J, et al.Immunity 2005 23:479-490. 2. Barksby HE, et al.C Exp Immunol 2007 149:217-225. 3. Arend WP, et al.ImmRev 2008 223:20-38. 4. Suzukawa M, et al.J. Immunol. 2008 181:5981-5989. 5. Moussio C, et al.PlosOne 2008 10:e3331.
Recombinant Mouse IL-33 (carrier-free), Recombinant, Il-1f11, Nuclear factor from high endothelial venules (NF-HEV), Immunology, Antibodies
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