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Recombinant Mouse IL-3 (carrier-free)
Recombinant Mouse IL-3 (carrier-free)
575502
10 µg
¥35,000
575504
25 µg
¥59,000
575506
100 µg
¥180,000
575508
500 µg
¥440,000
Product Details
Source:
Mouse IL-3, amino acids Ala 27 – Cys 166 (Accession# NM_010556), was expressed in E. coli.
Formulation:
0.22µm filtered protein solution is in 10mM NaHPO4 pH7.2, 0.15M NaCl
Endotoxin Level:
Less than 0.01ng per µg cytokine as determined by the LAL method.
Preparation:
10-100µg sizes are bottled at 200µg/ml. 500µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. Avoid repeated freeze/thaw cycles.
Activity:
ED50 = 0.100 ng /ml, corresponding to a specific activity of 1 x 107 units/mg, as determined by the dose dependent stimulation of a M-NFS-60 cell proliferation assay.
Bioactivity of mouse IL-3 was tested by a proliferation assay using M-NFS-60 cells.
Description:
IL-3 is the most potent growth factor for basophils followed by granulocyte-macrophage colony-stimulating factor and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and releasability. IL-3 is highly expressed by mast cells, and rapid and large amount of autocrine IL-3 production is responsible for mast cell survival by IgE in the absence of antigen. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma, atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis.
Activated T cells, Th1, Th2, mast cells, eosinophils, keratinocytes, NK cells, and endothelial cells.
Function:
Interleukine-3 is a pleiotropic factor which promotes both self-renewal and differentiation of early multipotential progenitors and is involved in inducible hematopoiesis in response to infections. IL-3 stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures.
Ligand Receptor:
Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR.
1. Kohno M, et al. 2005 Blood 105:2059-2065. 2. Valent P and Dahinden CA, et al. 2010 Curr Opin Hematol 17:60-66. 3. Kleemann R, et al. 2008 Cardiovascular Research 79:360-376. 4. Murphy JM and Young IG, et al. 2006 Vitam Horm 74:1-30.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
