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Recombinant Mouse IL-25 (IL-17E) (carrier-free)
Recombinant Mouse IL-25 (IL-17E) (carrier-free)
579802
10 µg
$195.00
579804
25 µg
$300.00
579806
100 µg
$600.00
579808
500 µg
$2,000.00
Product Details
Source:
Mouse IL-25, amino acids Val17-Ala169 (Accession # NM_080729) was expressed in E. coli.
Endotoxin Level:
Endotoxin level is <0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method.
Preparation:
10-100 µg sizes are bottled at 200 µg/mL. 500 µg sizes and larger are bottled at the concentration indicated on the vial.
Concentration:
0.22 μm filtered protein solution is in 20 mM NaHPO4 pH 6.0, 0.5M NaCl
Storage & Handling:
Unopened vial can be stored at 4°C for three months or -20°C to -70°C for six months. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. Stock solution can be stored up to 3 months from -20°C to -70°C under sterile conditions. Avoid repeated freeze/thaw cycles.
Activity:
Mouse IL-25 binds to recombinant mouse IL17BR/Fc Chimera in a functional ELISA.
Mouse IL-25 binding to recombinant IL17BR/Fc Chimera detected by functional ELISA
Description:
IL-25E (also known as IL-17E) is a member of the IL-17 family, which includes IL-17A, IL-17B, IL-17C, IL-17F and IL-17A/F. Primary sequence homology differs between family members, with hIL-17 and hIL-17F having the highest homology (44%), while hIL-17 and hIL-25 have the lowest (15%). IL-25 induces elevatedgene expression of IL-4, IL-5 and IL-13 in multiple tissues and results in T helper 2 (TH2)-type immune responses (increased serumIgE levels) and pathologic changes in the lungsand digestive tract with eosinophilic infiltrates, increased mucusproduction, epithelial cell hyperplasia and overall amplified allergicinflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B.
Other Names:
IL-17E
Structure:
Heterodimer
Distribution:
IL-25 is produced by mast cells, epithelial cells, alveolar macrophages, microglia and Th2 cells.
Function:
IL-25 directly promotes Th2 cell differentiation. IL-25 induces the expression of IL-4, IL-5, IL-13, and eotaxin and produces lung eosinophilia. In addition, IL-25 treatment of differentiated Th9 cells enhanced IL-9 production.
1. Hurst SD, et al. 2002. J. Immunol. 169:443. 2. Ikeda K, et al. 2003. Blood 101:3594. 3. Kang CM, et al. 2005. Am. J. Resp. Cell. Mol. 33:290. 4. Kleinschek ME, et al. 2007. J. Exp. Med. 204:161. 5. Terashima A, et al. 2008. J. Exp. Med. 205:2727. 6. Angkasekwinai P, et al. 2010. Nat. Immunol. 11:250.
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