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Recombinant Mouse IL-23 (carrier-free)
Recombinant Mouse IL-23 (carrier-free)
589002
10 µg
¥55,000
589004
25 µg
¥115,000
589006
100 µg
¥360,000
589008
500 µg
¥1,080,000
Product Details
Source:
The mouse IL-23 consists of two subunits linked via a disulphide bond: P19 (Accession# NM_031252: Met1-Ala 196) and P40 (Accession# NM_008352 : Met Met1-Ser 335). Mouse IL-23 was expressed in insect cells.
Formulation:
0.22 µm filtered protein solution is in PBS
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer containing carrier protein such as 1% BSA or HSA or 10% FBS.
Concentration:
The 10, 25, and 100 µg sizes are bottled at a concentration of 0.1 mg/ml. The 500 µg size is bottled at bulk concentration (see vial or CoA).
Storage & Handling:
Unopened vial can be stored at -20°C for six months or at -70°C for one year. After dilution, the cytokine can be stored at 4°C for one month or at -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
ED50=0.5 - 0.8 ng/ml, corresponding to a specific activity of 1.25-2.0 x 106 units/mg, as determined by mouse splenocytes IL-17A secretion induced by mIL-23 in a dose dependent manner. Under this assay, the bioactivity is equivalent
Induction of mouse IL-17A in splenocytes by mouse IL-23
Description:
IL-23 is a member of the IL-6 family of cytokines, and it is constituted by two subunits, p19 and p40. The p19-p40 heterodimer is stabilized by a disulfide bond. The subunit p40 is shared by IL-23 and IL-12 cytokines. p19 mRNA is expressed in endothelial cells and polarized T cells; nevertheless, p40 is not expressed by these cells. Therefore, the availability of functional IL-23 is limited by the expression of p40 and not p19. IL-23 exerts its biological activities through the interaction with a heterodimeric receptor complex composed of IL-12Rb1 and IL-23R. IL-23 activates Janus kinase (JAK)/signal transducer and activator of transcription signaling molecules (STAT). JAK2 is constitutively associated with the IL-23R chain, and binding of IL-23 to its receptor leads to phosphorylation of STAT1, STAT3, STAT4, and STAT5.
IL-23 is highly expressed by activated dendritic cells, macrophages, and epidermal Langerhans cells.
Function:
IL-23 promotes Th17 responses in vivo, and it is important for the survival and population expansion of Th17 cells. IL-23 induces proliferation of memory T cells and the induction of IL-17A and IL-17F in these cells. IL-23 induces the secretion of IL-17, IL-17F, IL-6, and TNFα in Th17 cells. In conjunction with IL-1, IL-23 is sufficient to induce naïve human T cells to produce IL-17A, IL-17F, IL-22, IL-26, IFN-γ, CCL20/MIP-3α and the transcription factor RORγt. PGE2 favors the production of IL-23 and inhibit IL-12 in dendritic cells. PGE2 synergizes with IL-23 in expanding Th17 cells from purified memory CD4+ T cells activated in the absence of accessory cells. IL-23 induces IFN-γproduction from T cells treated with PHA. In addition, IL-23 plays a key role in the pathogenesis of autoimmune and chronic inflammatory disorders. This role is supported by the identification of IL-23 receptor (IL-23R) susceptibility alleles associated with inflammatory bowel disease, psoriasis, and ankylosing spondylitis.
Ligand Receptor:
IL-23 binds to L-12Rb1 and IL-23R
Cellular Targets:
Memory T cells, Th17 cells, Inflammatory macrophages, NK cells, dendritic cells, and monocytes.
Antigen References:
1. Oppmann B, et al. 2000. Immunity 13:715. 2. Aggarwal S, et al. 2003. J. Biol. Chem. 278:1910. 3. Piskin G, et al. 2006. J. Immunol. 176:1908. 4. Ivanov II, et al. 2006. Cell 126:1121. 5. McGeachy MJ, et al. 2007. Nat. Immunol. 8:1390. 6. Wilson NJ, et al. 2007. Nat. Immunol. 8:950. 7. Bonifac B, et al. 2008. Immunol. Rev. 226:132. 8. Chizzolini C, et al. 2008. Blood 112:3696. 9. Buonocore S, et al. 2010. Nature 464:1371.
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