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Recombinant Mouse IL-2 (ELISA Standard)
Recombinant Mouse IL-2 (ELISA Standard)
575409 4 pack $80.00 Qty:
    

Product Details

 
Purity: >98%, as determined by SDS-PAGE, Coomassie stained with Invitrogen SimplyBlue SafeStain.
Formulation: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA.
Endotoxin Level: Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation: ELISA Standard 4-Pack contains four vials of recombinant mouse IL-2 at >5 ng per vial. Recommended for ELISA application only. Standards are calibrated against a BioLegend Master Calibrator.
Storage & Handling: Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application:

ELISA

Recommended Usage:

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 250 pg/ml to 2 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
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Application
Notes:

This IL-2 protein is useful as a standard for a mouse IL-2 sandwich ELISA, using unlabeled JES6-1A12 antibody (catalog # 503702) for capture and biotinylated JES6-5H4 antibody (catalog # 503804) for detection.




Sandwich ELISA Protocol




Description:
Cellular Sources: Mouse IL-2, amino acids Ala 21-Gln 169 (Accession # NM_008366) was expressed in E. coli.
GeneID: 16183
Latest Publications: View the latest IL-2 articles on HighwirePress.com
UniProt: View information about IL-2 on UniProt.org
Keywords: Recombinant Mouse IL-2 (ELISA Standard), Recombinant, IL-2 recombinant protein, recombinant IL-2 protein, ELISA, Immunology, Antibodies


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Product FAQs

 
How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
For a complete list of FAQs on all our products, visit our FAQ page.
 
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Related Categories

Category
Cytokines & Chemokines > Recombinant Proteins > Mouse > IL-2
Cytokines & Chemokines > Mouse Cytokine & Chemokine Research > Recombinant Proteins > IL-2
Cytokines & Chemokines > Mouse Cytokine & Chemokine Research > ELISA Antibody Pairs > IL-2

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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