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Recombinant Mouse IL-1β (carrier-free)
Recombinant Mouse IL-1β (carrier-free)
575102
10 µg
¥35,000
575104
25 µg
¥59,000
575106
100 µg
¥179,000
575108
500 µg
¥420,000
Product Details
Source:
Mouse IL-1β, amino acids Val118-Ser269 (Accession # NM_008361) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
Endotoxin Level:
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Preparation:
10-100µg sizes are bottled at 200µg/mL. 500µg sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity:
ED50 =0.001 -0.005 ng/ml, corresponding to a specific activity of 1- 0.2 x 109 units/mg, as determined by the dose dependent stimulation of D10S cells proliferation
Use when high specific biological activity is required.
COA:
Application Notes:
This IL-1β protein is biologically active, and can be used for in vitro assays
Description:
Interleukin-1 is a key mediator of inflammation, with pleiotropic effects on several cells and signaling pathways. The activity defined as IL-1 reflects the function of 2 molecules, IL-1α and IL-1β. IL1A encodes IL-1α, which is cell-bound, and IL1B encodes IL-1β, a secreted cytokine (1). IL-1α and IL-1β are synthesized as 31-kD precursors and are processed by proteases to their mature 17-kD forms. IL-1β-converting enzyme cleaves the inactive IL-1β Precursor and ProIL-1α is processed by calpain (4). IL-1β is a tumor-promoting cytokine, and it enhances tumor metastasis and angiogenesis. IL-1β is able to facilitate tumor progression in murine models of lung cancer. Upregulation of metastasis and tumor angiogenesis by IL-1β has been associated with increased activity of matrix metalloproteinases and expression of the pro-angiogenic molecule hepatocyte growth factor (5).
Other Names:
Il-1b, IL-1beta
Structure:
Cytokine
Distribution:
IL-1β is produced by monocytes, macrophages, keratinocytes, stimulated B lymphocytes, fibroblasts, endothelial cells, and epithelial cells.
Ligand Receptor:
Type I and II IL-1 receptors (IL-1RI and IL-1RII)
Antigen References:
1. Johnsen AK, et al.Arthritis Rheum 58:1947-1957 2008. 2. Brinster C and Shevach EM J. Leukoc. Biol. 84:480-487 2008. 3. O'Sullivan BJ, et al.J. Immunol. 176:7278-7287 2006. 4. Nazarenko I, et al.Neoplasia 10:549-562 2008. 5. Boost KA, et al.BMC Cancer 8:265 2008.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
