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Recombinant Mouse IL-1α (carrier-free)
Recombinant Mouse IL-1α (carrier-free)
575002
10 µg
¥35,000
575004
25 µg
¥59,000
575006
100 µg
¥159,000
575008
500 µg
¥420,000
Product Details
Source:
Mouse IL-1α, amino acids Ser115-Ser270 (Accession# NM_010554) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
Endotoxin Level:
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Preparation:
10-100µg sizes are bottled at 200µg/mL. 500µg sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity:
ED50 =1- 5 pg/ml, corresponding to a specific activity of 1- 0.2 x 109 units/mg, as determined by the dose dependent stimulation of D10S cells proliferation
Use when high specific biological activity is required.
COA:
Application Notes:
This IL-1α protein is biologically active, and can be used for in vitro assays
Description:
IL-1 was isolated from human blood that had been exposed to a pathogenic bacterium. IL-1 is a pyrogen, and it is an activating factor for lymphocytes. It also damaged joints and influenced liver proteins (3). IL-1α binds to the cell surface type I and II IL-1 receptors (IL-1RI and IL-1RII). IL-1 and -β and IL-1RA can compete for binding to these receptors. However, only IL-1RI, not IL-1RII, is functional because IL-1RII lacks a cytoplasmic domain and is thus unable to transmit signals to downstream steps (4). During ovarian inflammatory response, proinflammatory cytokine production such as IL-1 is augmented in granulosa cells and can induce local chemokine synthesis, which in turn may affect ovarian function (1). Also, IL-1 is involved in regulating tissue chemokine expression and leukocyte accumulation. The abundant influx of leukocytes into the ovary varies with the stage of the cycle and the leukocytes are thought to have a central role in influencing follicular atresia, ovulation, and luteal function and are potentially involved in ovarian disorders such as premature ovarian failure and polycystic ovary syndrome (1). IL-1a induces CXCl1 RNA and protein in mouse granulose cells.
Other Names:
RP23-160G19.8, Il-1a
Structure:
Cytokine
Distribution:
IL-1α is produced by monocytes, macrophages, keratinocytes, stimulated B lymphocytes, fibroblasts, endothelial cells, and epithelial cells. In addition, IL-1α is localized in various ovarian cell types such as the oocyte, granulosa, and theca cells, and ovarian cancer cells (1).
Function:
The cytokine IL-1α is a key molecule both in the innate and in the adaptive immune response. IL-1α induces autonomous proliferation in vascular smooth muscle cells, T cell activation during Ag presentation, up-regulation of monocyte/macrophage-mediated tumor cytotoxicity, stimulation of osteoclast formation, and expression of Cxcl1 (kC) chemokine mRNA and protein in mouse granulosa cells (1,2).
Ligand Receptor:
Type I and II IL-1 receptors (IL-1RI and IL-1RII)
Antigen References:
1. Soo DS and Roby KF, et al. Mol Endocri 20:2999-3013 2006. 2. Yatabe T, et al.Ann Rheum Dis published online 28 Jul 2008. 3. Ledford H et al, Nature 450:29-30 2007. 4. Boraschi B Tagliabue A et al, Vitam Horm 74:229-254 2006.
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