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Recombinant Mouse IL-17F (carrier-free)
Recombinant Mouse IL-17F (carrier-free)
576106
100 µg
$550.00
576108
500 µg
$1,550.00
Product Details
Source:
Mouse IL-17F, amino acids Arg29-Ala161 (Accession # NM_145856) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in 10 mM NaH2PO4, 0.15 M NaCl, pH 7.2.
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
10 to 100 µg sizes are bottled at 200 µg/mL, 500 µg sizes and larger are bottled at the concentration indicated on the vial.
Concentration:
Storage & Handling:
Unopened vial can be stored at 4°C for three months or -20°C to -70°C for six months. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity:
ED50 =300 to 600 ng/ml, corresponding to a specific activity of 0.16 - 0.33 x 104 units/mg, as determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of CXCL1.
1. Gaffen, S.L., et al., 2006. Vit Horm. 74:255. 2. Oda, N., et al., 2005. Am. J. Respir. Crit. Care Med. 171:12. 3. Yang, XO, 2008. J. Exp. Med. 205:1063. 4. Ishigame, H., et al., 2009. Immunity 30:108. 5. Kuestner, R.E., 2007. J. Immunol. 179:5462. 6. Tan, W., et al., 2008. Experimental Hematol. 36:1417. 7. Nagata, T., et al., 2008. J. Immunol. 181:7473.
CXCL1 induced by mouse IL-17F in fetal mouse skin fibroblasts
Description:
IL-17F belongs to the IL-17 cytokine family which includes IL-17A, B, C, D, and E (also called IL-25). IL-17F shares the strongest homology to IL-17A. They share 50% amino acid sequence homology. Recently, an IL-17–IL-17F heterodimer was found to be expressed in Th17 cells together with IL-17 and IL-17F homodimers. IL-17 and IL-17F gene promoters share the same pattern of chromatin remodeling in differentiated Th17 cells. T cell populations in vitro and in vivo have shown different ratios of IL-17 and IL-17F expression. IL-17, IL-17F regulates proinflammatory gene expression in vitro. Studies in vivo using Il17a(-/-), Il17f(-/-), and Il17a(-/-)Il17f(-/-) mice showed that IL-17F played only marginal roles, if at all, in the development of delayed-type and contact hypersensitivities, autoimmune encephalomyelitis, collagen-induced arthritis, and arthritis in Il1rn(-/-) mice. In contrast, both IL-17F and IL-17A were involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium. Additional studies in vivo have suggested a role for IL-17F in the induction of neutrophilia in the lungs and in the exacerbation of Ag-induced pulmonary inflammation.
Other Names:
Interleukin-17F
Structure:
Cytokine
Distribution:
T cells, Th17 cells, memory CD4 T cells, innate immune cells, and epithelial cells.
Ligand Receptor:
IL-17RC, IL-17F can signal via the IL-17RA; however, with lower affinity than IL17A.
Interaction:
IL-17F might act primarily on parenchymal cells such as fibroblasts, epithelial cells, and endothelial cells.
Cellular Sources:
T cells, Th17 cells, memory CD4 T cells, innate immune cells, and epithelial cells.
Receptors:
IL-17RC, IL-17F can signal via the IL-17RA; however, with lower affinity than IL17A.
Bioactivity/Activities:
IL-17F induces IL-6 and KC (CXCL1) production in mouse fibroblast and macrophages. IL-17F/IL-17R interaction stimulates granulopoiesis in mice. IL-17F homodimer via IL-17RA is a negative regulator of IL-17 production in T cells.
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