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Recombinant Mouse IL-15 (carrier-free)
Recombinant Mouse IL-15 (carrier-free)
566301
2 µg
¥15,000
566302
10 µg
¥45,000
566304
100 µg
¥179,000
Product Details
Source:
Mouse IL-15, amino acids Asn49-Ser162 (Accession # NM_008357) was expressed in E. coli.
Formulation:
Lyophilized from a concentrated solution (1 mg/ml) with 10 mM NaH2PO4, 150 mM NaCL, pH 8.0.
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer containing carrier protein such as 1% BSA or HSA or 10% FBS.
Storage & Handling:
Unopened vial can be stored from -20°C to -70°C for 12 months. Reconstituted protein can be stored for one month at 4°C or up to three months from -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity:
ED50=2.5 - 7.5 ng/ml, corresponding to a specific activity of 4 - 1.3 x 105 units/mg, as determined by the dose dependent stimulation of CTLL-2 cell proliferation.
This IL-15 protein is biologically active and can be used for in vitro assays.
CTLL-2 cell proliferation induced by mouse IL-15
Description:
IL-15 was discovered in the supernatant of Simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2. IL-15 is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, fibroblasts, and monocytes. IL-15 plays an important role in immune response and shares many functions with IL-2. For example, it stimulates the proliferation of activated T cells, NK cells, and B cells, and induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells and induces the production of proinflammatory cytokines from macrophages. IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system.
IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares the receptor chains β and γc with IL-2. IL-15 is normally not secreted in soluble form but is instead held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 is then presented in trans form to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and intermittent proliferation.
Other Names:
IL-15
Structure:
Cytokine
Distribution:
IL-15 is expressed by DC, epithelial cells, fibroblasts, and monocytes.
Function:
IL-15 stimulates the proliferation of activated T cells, NK cells, and B cells, and inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells, and induces the production of proinflammatory cytokines from macrophages.
Ligand Receptor:
IL-15Rα, IL-15Rβ and IL-15γc
Antigen References:
1. Grabstein K, et al. 1994. Science 264:965. 2. Ma A, et al. 2006. Annu. Rev. Immunol. 24:657. 3. Meresse B, et al. 2004. Immunity 21:357. 4. Armitage RJ, et al. 1995. J. Immunol. 154:483. 5. Pulendran B, et al. 2004. Eur. J. Immunol. 34:66. 6. Bouchard A, et al. 2004. J. Leukoc. Biol. 76:162. 7. Ratthe C, et al. 2004. J. Leukoc. Biol. 75:893. 8. Feng T, et al. 2008. Cell. Immunol. 5:189. 9. Rubinstein MP, et al. 2006. P. Natl. Acad. Sci. USA 103:9166.
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