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Recombinant Mouse G-CSF (carrier-free)
Recombinant Mouse G-CSF (carrier-free)
574602
10 µg
¥37,000
574604
25 µg
¥85,000
574606
100 µg
¥205,000
574608
500 µg
¥600,000
Product Details
Source:
Mouse G-CSF, amino acids Val31-Ala208 (Accession# NM_009971) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in PBS.
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
10-100 µg sizes are bottled at 200 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
ED50 =0.5 - 3 ng/ml, corresponding to a specific activity of 0.33 - 2.0 x 106 units/mg, as determined by M-NFS-60 cell proliferation induced by mouse G-CSF in a dose dependent manner.
M-NFS-60 cell proliferation induced by mouse G-CSF.
Description:
G-CSF is the key hematopoietic cytokine involved in the control of neutrophil production; therefore it is a critical regulator of innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by down-regulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophil and monocyte proteolytic enzymes, including neutrophil elastase, cathepsin G, and matrix metalloproteinase-9, which in turn degrade important HSC-trafficking and adhesion molecules c-kit, VCAM-1, and CXCR4. In addition, G-CSF possesses immunosuppressive effects on monocytes/ macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells; in addition, it is expressed in cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells.
Monocytes, mesothelial cells, fibroblasts, endothelial cells, and neurons.
Function:
G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation; on mature neutrophils, G-CSF enhances survival, superoxido anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
Ligand Receptor:
G-CSFR.
Interaction:
Hematopoietic progenitor cells, neutrophilic granulocytes, monocytes, platelets, endothelial cells, trophoblastic cells, myoblasts, cardiomyocytes, neurons, and some small cell lung carcinoma cell lines.
Antigen References:
1. Kim HK, et al. 2006. Blood 108:812. 2. Donahue RE, et al. 2009. Blood 114:2530. 3. Shimoji K, et al. 2010. Cell. Stem. Cell. 6:227. 4. Martins A, et al. 2010. IUBMB Life 62:611. 5. Hara M, et al. 2011. J. Exp. Med. 208:715.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
