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Recombinant Mouse CXCL9 (MIG) (carrier-free)
Recombinant Mouse CXCL9 (MIG) (carrier-free)
578202
10 µg
¥25,000
578204
25 µg
¥45,000
578206
100 µg
¥119,000
578208
500 µg
¥400,000
Product Details
Source:
Mouse CXCL9, amino acids Thr22 - Thr126 (Accession # NM_008599) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in PBS
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
10-100 µg sizes are bottled at 200 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to three months. Avoid repeated freeze/thaw cycles.
Activity:
Bioactivity was measured by its property to chemoattract human T cells activated with PHA and IL-2 in a dose dependent manner.
CXCL9 is an inflammatory chemokine initially identified by differential screening of a cDNA library from limphokine-activated macrophages. CXCL9 is a CXC chemokine and member of the non-ELR (lacking a Glu-Leu-Arg motif in the N-terminal region) CXC chemokine family. CXCL9 shares the CXCR3 receptor with CXCL10 and CXCL11, and these ligands differ in their receptor-binding and activating properties; CXCL11 is more potent than CXCL10 and CXCL10 is more potent than CXCL9.
Other Names:
MIG; Monokine induced by interferon gamma; SCYB9; Small inducible cytokine, subfamily B, member 9
Structure:
Chemokine
Distribution:
CXCL9 is secreted by monocytes, macrophages, APC, eosinophils, endothelial cells, and B cells. Also, CXCL9 is secreted by fibroblast in inflammatory conditions.
Function:
CXCL9 plays a key role in leukocyte trafficking, acting on activated CD4+ Th1 cells, CD8+ T cells, IL-2 activated T lymphocytes, and NK cells. In addition, CXCL9 enhances T lymphocyte function in alloimmune response; CXCL9 induces T cells proliferation and cytokine production in an experimental model of cardiac allograft vasculopathy. Also, CXCL9 has angiostatic activity. Additionally, it has been hypothesized that CXCL9, CXCL10, and CXCL11 contribute to host defense against inhalational anthrax in a murine model of infection. CXCL9 is induced by cytokines, particularly IFNγ during infection, injury, or immunoinflammatory responses, and it can be inactivated by CD26/dipeptidil peptidase IV after truncation by this protease.
Ligand Receptor:
CXCR3
Interaction:
Activated T lymphocytes, CD4 Th1, CD8+, NK, and NKT cells.
Antigen References:
1. Loetscher M, et al. 1996 J. Exp. Med. 184:963. 2. Lambeir AM, et al. 2001. J. Biol. Chem. 276:29839. 3. Whiting D, et al. 2004 J. Immunol. 172:7417. 4. Gorbachev AV, et al. 2007. J. Immunol. 178:2278. 5. Rosenblum JM, et al. 2010. J. Immunol. 184:3450. 6. Crawford MA, et al. 2010 PLoS Pathog 6:e1001199.
Recombinant Mouse CXCL9 (MIG) (carrier-free), Recombinant, MIG; Monokine induced by interferon gamma; SCYB9; Small inducible cytokine, subfamily B, member 9, Immunology, Antibodies
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