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Recombinant Mouse CXCL10 (IP-10) (carrier-free)
Recombinant Mouse CXCL10 (IP-10) (carrier-free)
573602
10 µg
¥19,000
573604
25 µg
¥38,000
Product Details
Source:
Mouse CXCL10, amino acids Ile22-Pro98 (Accession# NM_021274) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in PBS.
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
10 and 25 µg sizes are bottled at 200 µg/mL.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
Bioactivity was measured by its property to chemoattract PHA, IL-2 activated T cells in a dose dependent manner.
CXCL10 is an ELR-negative chemokine structurally and functionally related to CXCL9 and CXCL11. CXCL10, CXCL9, and CXCL11 are produced and secreted by monocytes, macrophages, fibroblasts, and epithelial cells upon stimulation with proinflammatory cytokines, especially IFNγ. CXCL10 chemoattracts CD4, CD8, and NK and NKT cells through the binding to its receptor CXCR3, which is shared with CXCL9 and CXCL11. In addition, CXCL10 inhibits neovascularization in tumors and in wound healing in vivo. Also, CXCL10 has anti-proliferative effects on endothelial cells in vitro, and angiostatic and antitumor effects in vivo. It has been suggested that the anti-proliferative effect of CXCL10 in endothelial cells is CXCR3-independent and that it is mediated through GAG interaction. CXCL10 also possesses antimicrobial activity against E. coli and L. monocytogenes, and both the spore and bacillus forms of B. anthracis. CXCL10 expression is strongly upregulated in many inflammatory diseases, including arthritis, type I diabetes, experimental autoimmune encepahlomyelitis, atherosclerosis, allograft rejection, and others.
Interferon gamma-stimulated keratinocytes, monocytes, macrophages, fibroblasts, endothelial, and T cells.
Function:
CXCL10 chemoattracts CD4, Th1, CD8, and NK and NKT cells. CXCL10 is induced by inflammatory cytokines. NH2-terminal cleavage of CXCL10 by DPP-IV/CD26 impairs its chemoattracting capacity and CXCR3 signaling ability.
Ligand Receptor:
CXCR3
Interaction:
Activated T cells, Th1 cells, regulatory T cells, NK cells, NKT cells, endothelial cells, and fibroblasts.
Antigen References:
1. Luster AD, et al. 1985. Nature 315:672. 2. Vanguri P, et al. 1990. J. Biol. Chem. 265:15049. 3. Loos T, et al. 2008. Blood 112:2648. 4. Crawford MA, et al. 2009. Infect. Immun. 77:1664. 5. Campanella GS, et al. 2010. PLOS One 5:e12700. 6. Hoerning A. 2011. Eur. J. Immunol. 41:2291.
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