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Recombinant Mouse CXCL1 (KC) (carrier-free)
Recombinant Mouse CXCL1 (KC) (carrier-free)
573702
10 µg
¥30,000
573704
25 µg
¥65,000
573706
100 µg
¥200,000
573708
500 µg
¥520,000
Product Details
Source:
Mouse CXCL1, amino acids Asn29-Lys96 (Accession# NM_008176.3) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in PBS.
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
10-100 µg sizes are bottled at 200 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
Bioactivity was measured by its property to chemoattract human neutrophils in a dose dependent manner.
The CXCL1/KC gene was initially identified as being regulated by PDGF in BALB/c-3T3 cells. CXCL1/KC is an ELR CXC chemokine, and it is structurally and functionally related to the human GROs (CXCL1-CXCL3) and interleukin-8 (CXCL8). CXCL1, CXCL2, and CXCL3 bind to the CXCR2 receptor, with CXCL1 having the highest affinity. CXCL1 is crucial for the recruitment of neutrophils to inflammatory sites. In addition, CXCL1 has pleiotropic effects on cell proliferation, tumor angiogenesis, invasion, and metastasis. CXCL1 and CXCL2 induce neutrophil mobilization from the bone marrow in a murine model of acute peritonitis, and CXCL1 and G-CSF act cooperatively in this process. In addition, CXCL1 mediates murine sickle cell vasoocclusion during hemolytic transfusion reactions. Thrombin enhances the secretion of CXCL1 from tumor cells, and PGE2 stimulates expression of CXCL1 in human colorectal carcinoma cells (CRC). Furthermore, CXCL1 (secreted by CRC cells) induces endothelial cell migration and tube formation in vitro and in vivo. CXCL1 is induced by inflammatory cytokines such as IL-1 and TNF, and IL-17A enhances expression of CXCL1 by prolonging the half-life of its constitutively unstable mRNA.
Other Names:
KC, Secreted protein N51, FSP, Grow regulated oncogen 1 (GRO1), Grow regulated oncogen a (GRO a), Melanoma growth stimulating activity (MGSA), MGSA-a, neutrophil activating protein 3 (NAP-3), SCYB1
Structure:
Chemokine
Distribution:
CXCL1 is produced by neutrophils, activated monocytes, macrophages, fibroblasts, keratinocytes, epithelial cells, and endothelial cells.
Function:
CXCL1 chemoattracts neutrophils, and induces cell proliferation, tumor angiogenesis, invasion, and metastasis. CXCL1 is induced by IL-1, TNF, thrombin, and PGE2.
Ligand Receptor:
CXCR2
Interaction:
Neutrophils and endothelial cells
Antigen References:
1. Cochran BH, et al. 1983. Cell 33:939. 2. Anisowicz A, et al. 1987. P. Natl. Acad. Sci. USA 84:7188. 3. Caunt M, et al. 2006. Cancer Res. 66:4125. 4. Wang D, et al. 2006. J. Exp. Med. 203:941. 5. Wengner AM, et al. 2008. Blood 111:42. 6. Stillie R, et al. 2009. J. Leukoc. Biol. 86:529. 7. Datta S, et al. 2010. J. Immunol. 184:1484. 8. Jang JE, et al. 2011. J. Clin. Invest. 121:1397.
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