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Recombinant Mouse CCL2 (MCP-1) (carrier-free)
Recombinant Mouse CCL2 (MCP-1) (carrier-free)
576502
10 µg
¥39,000
576504
25 µg
¥70,000
Product Details
Source:
Mouse MCP1, amino acids Gln24-Arg96 (Accession # NM_011333) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in 10mM NaHPO4 pH 7.2, 0.15M NaCl
Endotoxin Level:
Less than 0.01ng per µg cytokine as determined by the LAL method.
Preparation:
10 - 100 µg sizes are bottled at 200 µg/mL. 500 µg sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, DPBS, HBSS, or EBSS) containing carrier protein such as 1% BSA or HSA. For long-term storage, aliquot into polypropylene vials and store in a manual defrost freezer. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
CCL2, also known as MCP-1, is a member of the CC ß chemokine family. It is widely expressed in endothelial cells, smooth muscle cells and monocytes in response to several atherogenic stimulants such as CD40 ligand, platelet derived growth factor (PDGF), interleukin-1β (IL-1β) and oxidized low density lipoprotein. Several recent in vivo studies have disclosed critical roles of MCP1 in atherosclerosis. In addition, MCP-1 has been implicated in monocytic infiltration of tissues during several inflammatory diseases, and has been implicated in macrophage-mediated tumor growth suppression in mice. CCL2 has been shown to have direct effects on tumor cells in an autocrine and paracrine fashion in multiple cancers, including breast, lung, cervix, ovary, sarcoma, and prostate. In addition, MCP-1 plays a key role in the regulation of MMPs during transmigration. Ccl2 induces migration of MonoMac6 by induction of MMP2. MCP-1 and Ccl3/MIP-1alpha mediate firm adherence and (subsequent) transmigration of neutrophils via protein synthesis and secondary generation of leukotrienes and PAF, which in turn directly activate neutrophils in mouse cremaster muscle. MCP-1 is induced from endothelial cells by MIF and from fetal skin fibroblast by IL-17.
Other Names:
Monocyte chemotactic protein 1, chemokine (C-C motif) ligand 2, Ccl2, JE, Scya2, monocyte chemotactic and activating factor, MCAF.
MCP-1 induces migration of monocytes, memory/activated T cells, NK cells, myeloid dendritic cells, neutrophils, astrocytes, mesangial cells, and bone marrow endothelial cells. MCP-1 is induced by IL-1b, TNF, IFNg, PDGF, IL-4, MIF, and IL-17.
Ligand Receptor:
MCP-1 mediates its cellular effects primarily through CCR2 receptor
Bioactivity/Activities:
ED50 = 8 -15 ng/ml, corresponding to a specific activity of 1.25 – 0.6 x 105 units/mg, as determined by the dose dependent chemoattraction of THP-1 cells
Antigen References:
1. Loberg RD, et al. 2007 Cancer Research 67:9417-9424. 2. Gregory JL, et al. 2006 J Immunol 177:8072-8079. 3. Qui Z, et al. 2009 Immunology 214:835-842. 4. Reichel CA, et al. 2009 Arterioscler Thromb Vasc Biol. ATVBAHA.109.193268v1 5. McQuibban GA, et al. 2002 Blood 100:1160-1167
Recombinant Mouse CCL2 (MCP-1) (carrier-free), Recombinant, Monocyte chemotactic protein 1, chemokine (C-C motif) ligand 2, Ccl2, JE, Scya2, monocyte chemotactic and activating factor, MCAF., Immunology, Antibodies
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