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Recombinant Human TNF-α (ELISA Std.)
Recombinant Human TNF-α (ELISA Std.)
570109
4 pack
¥16,000
Product Details
Source:
Human TNF-alpha, amino acids Val77-Leu233 (Accession # HUMTNFAB) was expressed in E. coli.
Formulation:
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA.
Endotoxin Level:
Less than 0.01ng per µg cytokine as determined by the LAL method.
Preparation:
ELISA Standard 4-Pack contains four vials of recombinant human TNF-α at >5 ng per vial. Recommended for ELISA application only. Standards are calibrated against a BioLegend Master Calibrator.
Storage & Handling:
Upon receipt, store unopened vials at 4°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA: This TNF-α protein is useful as a standard for a human TNF-α sandwich ELISA, using unlabeled MAb1 antibody (Cat. No. 502802) for capture and biotinylated MAb11 antibody (Cat. No. 502904) for detection.
Description:
TNF-α is released from macrophages, monocytes, neutrophils, T-cells (principally CD4+), NK-cells and many transformed cell lines. Soluble homotrimeric TNF-α is released from cells by proteolysis of the integral membrane precursor form of TNF-α. TNF-α binding to some TNF-α receptors induces apoptosis and depending on cell type, receptor expression, and signal transduction status can induce other responses. TNF-α is involved in the inflammatory response.
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,
Cellular Sources:
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
