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Recombinant Human PDGF-BB (carrier-free)
Recombinant Human PDGF-BB (carrier-free)
577302
10 µg
¥25,000
577304
25 µg
¥45,000
577306
100 µg
¥144,000
577308
500 µg
¥360,000
Product Details
Source:
Human PDGF-BB, amino acids Ser82-Thr190 (NM_033016) was expressed in E. coli.
Formulation:
0.22µm filtered protein solution is in 29% acetonitrile, 0.1% TFA (trifluoroacetic acid).
Endotoxin Level:
Less than 0.01ng per µg cytokine as determined by the LAL method.
Preparation:
10-100 µg sizes are bottled at 200 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at -20°C for six months or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term-storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity:
ED50 = 10-20 ng/ml, corresponding to a specific activity of 1-0.5 x 105 units/mg, as determined by the dose dependent stimulation of 3T3 cell proliferation. The bioactivity is equivalent to competitor reported values.
This PDGF-BB protein is biologically active, and can be used for in vitro assays
Application References:
1. Banerjee, P., et al. 2011. J. Biol Chem. 23:33580. PubMed.
Description:
PDGF-BB is a mitogen initially identified as simian sarcoma viral oncogene homolog. It belongs to the PDGF family which includes five members, PDGF-AA, PDGF-BB, PDGF-AB, PDGFCC, and PDGF-DD. PDGF-AA, AB, and BB dimers are processed intracellularly and secreted as active dimers that readily activate PDGF receptors (PDGFRs). PDGF CC and DD are secreted as full-length, latent dimers, and the proteolytic removal of a CUB domain is required for the growth factor domain of PDGF CC or DD to activate the PDGF receptors. PDGF-BB exerts its biological functions through the activation of dimeric receptors made up of two structurally similar protein-tyrosine kinase receptor subunits (αα-, αβ-, or ββ-PDGFR). PDGF has been characterized as a chemoattractant for vascular smooth muscle cells from both sheep and humans and also for canine tracheal myocytes. In cutaneous remodelling, studies ex vivo revealed that PDGF-BB is a mitogen and chemoattractant for dermal fibroblast. Abnormalities of PDGFR/PDGF are thought to contribute to a number of human diseases, and especially malignancy. Autocrine signalling as a consequence of PDGF-B overexpression is clearly implicated in the pathogenesis of dermatofibrosarcoma protruberans (DFSP).
PDGF-B is mainly expressed in vascular endothelial cells, megakaryocytes, and neurons.
Function:
PDGF-BB regulates diverse cellular processes, including cell proliferation, transformation, migration, survival, and apoptosis of mesenchymal cells, in development and during pathogenesis.
Ligand Receptor:
αα-PRGFR, αβ-PDGFR, and ββ-PDGFR
Interaction:
Mesenchymal-derived cells
Antigen References:
1. Carlin SM, et al. 2003 Am J Physiol Lung Cell Mol Physiol 284:L1020-L1026. 2. Ustach CV, et al. 2005 Mol Cell Biol 25:6279-6288. 3. Jones AV, Cross NC, et al. 2004 Cell Mol Life Sci. 61:2912-2923. 4. Andrae J, et al. 2008 Genes Dev. 22:1276-1312.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
