Human PDGF-BB, amino acids Ser82-Thr190 (NM_033016) was expressed in E. coli.
Formulation:
0.22µm filtered protein solution is in 29% acetonitrile, 0.1% TFA (trifluoroacetic acid).
Endotoxin Level:
Less than 0.01ng per µg cytokine as determined by the LAL method.
Storage & Handling:
Unopened vial can be stored at -20°C or -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term-storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity:
ED50 = 10-20 ng/ml, corresponding to a specific activity of 1-0.5 x 105 units/mg, as determined by the dose dependent stimulation of 3T3 cell proliferation. The bioactivity is equivalent to competitor reported values.
Application:
BA, Blocking
Application Notes:
This PDGF-BB protein is biologically active, and can be used for in vitro assays
Description:
PDGF-BB is a mitogen initially identified as simian sarcoma viral oncogene homolog. It belongs to the PDGF family which includes five members, PDGF-AA, PDGF-BB, PDGF-AB, PDGFCC, and PDGF-DD. PDGF-AA, AB, and BB dimers are processed intracellularly and secreted as active dimers that readily activate PDGF receptors (PDGFRs). PDGF CC and DD are secreted as full-length, latent dimers, and the proteolytic removal of a CUB domain is required for the growth factor domain of PDGF CC or DD to activate the PDGF receptors. PDGF-BB exerts its biological functions through the activation of dimeric receptors made up of two structurally similar protein-tyrosine kinase receptor subunits (αα-, αβ-, or ββ-PDGFR). PDGF has been characterized as a chemoattractant for vascular smooth muscle cells from both sheep and humans and also for canine tracheal myocytes. In cutaneous remodelling, studies ex vivo revealed that PDGF-BB is a mitogen and chemoattractant for dermal fibroblast. Abnormalities of PDGFR/PDGF are thought to contribute to a number of human diseases, and especially malignancy. Autocrine signalling as a consequence of PDGF-B overexpression is clearly implicated in the pathogenesis of dermatofibrosarcoma protruberans (DFSP).
PDGF-B is mainly expressed in vascular endothelial cells, megakaryocytes, and neurons.
Function:
PDGF-BB regulates diverse cellular processes, including cell proliferation, transformation, migration, survival, and apoptosis of mesenchymal cells, in development and during pathogenesis.
Ligand Receptor:
αα-PRGFR, αβ-PDGFR, and ββ-PDGFR
Interaction:
Mesenchymal-derived cells
Antigen References:
1. Carlin SM, et al., 2003, Am J Physiol Lung Cell Mol Physiol 284: L1020-L1026. 2. Ustach CV, et al., 2005 Mol Cell Biol 25:6279-6288. 3. Jones, AV., Cross, NC. et al., 2004, Cell Mol Life Sci. 61:2912-2923. 4. Andrae J., et al., 2008, Genes Dev. 22: 1276-1312.
