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Recombinant Human CCL2 (MCP-1) (carrier-free)
Recombinant Human CCL2 (MCP-1) (carrier-free)
571402 10 µg $125.00 Qty:
    
571404 25 µg $205.00 Qty:
    
571406 100 µg $595.00 Qty:
    
571408 500 µg $1,500.00 Qty:
    

Product Details

 
Source: Human MCP1, amino acids Gln24-Thr99 (NM_002982) was expressed in E. coli.
Molecular Mass: The 76 amino acid recombinant protein has a predicted molecular mass of approximately 8,685 Da. The DTT-reduced protein migrates at approximately 8kDa and non-reduced protein migrates at approximately 13kDa by SDS-PAGE. The N-terminal amino acid is Glycine.
Purity: Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation: 0.22 µm filtered protein solution is in 10mM NaHPO4 pH 7.2, 0.15M NaCl
Endotoxin Level: Less than 0.01ng per µg cytokine as determined by the LAL method.
Preparation: 10-100µg sizes are bottled at 200µg/mL. 500µg sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, DPBS, HBSS, or EBSS) containing carrier protein such as 1% BSA or HSA. For long-term storage, aliquot into polypropylene vials and store in a manual defrost freezer. After dilution, the cytokine can be stored between 2°C and 8°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity: ED50 = 6-15 ng/ml, corresponding to a specific activity of 1.6-0.6 x 105 units/mg, as determined by the dose dependent chemoattraction of THP-1 cells
Application:

Bioassay

COA:
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Recombinant Human CCL2 (MCP-1) (carrier-free)  Image 1
THP-1 cell chemotaxis induced by human MCP-1.








Description:

CCL2, also known as MCP-1, is a member of the CC ß chemokine family. It is widely expressed in endothelial cells, smooth muscle cells and monocytes in response to several atherogenic stimulants such as CD40 ligand, platelet derived growth factor (PDGF), interleukin-1β (IL-1β) and oxidized low density lipoprotein. Several recent in vivo studies have disclosed critical roles of MCP1 in atherosclerosis. In addition, MCP-1 has been implicated in monocytic infiltration of tissues during several inflammatory diseases, and has been implicated in macrophage-mediated tumor growth suppression in mice. CCL2 has been shown to have direct effects on tumor cells in an autocrine and paracrine fashion in multiple cancers, including breast, lung, cervix, ovary, sarcoma, and prostate. In addition, MCP-1 plays a key role in the regulation of MMPs during transmigration.MCP-1/CCl2 has been described as a new diagnostic marker and therapeutic target for progressive renal injury in diabetic nephropathy. Kidney epithelial cells, including glomerular podocytes and tubular cells make MCP-1 in response to high glucose and advanced glycation end products. MCP-1 promotes inflammation and progressive injury in diabetic kidneys. The importance of MCP-1 in the early development of diabetic nephropathy has been determined in animal models incorporating genetically deficient mice or therapeutic blockade of MCP-1 receptor (CCR2).

Other Names: Monocyte chemotactic and activating factor (MCAF); monocyte secretory protein JE; small inducible cytokine A2 (SCYA2)
Structure: Chemokine
Distribution: MCP-1 (CCL2) is secreted by fibroblast, endothelial cells, smooth muscle cells, tumor cells, phytohemaglutinin stimulated mononuclear cells.
Function: MCP-1 induces migration of monocytes, memory/activated T cells, NK cells, myeloid dendritic cells, neutrophils, astrocytes, mesangial cells, and bone marrow endothelial cells. MCP-1 is induced by IL-1b, TNF, IFNg, PDGF, IL-4, MIF, and IL-17.
Ligand Receptor: MCP-1 mediates its cellular effects primarily through CCR2 receptor
Antigen
References:

1. Loberg RD, et al. 2007. Cancer Research. 67:9417-9424.
2. Gregory JL, et al. 2006. J. Immunol. 177:8072-8079.
3. Qui Z, et al. 2009. Immunology. 214:835-842.
4. Reichel CA, et al. 2009. Arterioscler Thromb Vasc Biol. ATVBAHA.109.193268v1
5. McQuibban GA, et al. 2002. Blood 100:1160-1167
5. Tesh GH, et al. 2008. Amm J Physiol Renal Physiol. 294:F697-F701.

GeneID: 6347
Latest Publications: View the latest CCL2 articles on HighwirePress.com
UniProt: View information about CCL2 on UniProt.org
Keywords: Recombinant Human CCL2 (MCP-1) (carrier-free), Recombinant, CCL2 recombinant protein, recombinant CCL2 protein, Monocyte chemotactic and activating factor (MCAF); monocyte secretory protein JE; small inducible cytokine A2 (SCYA2), Bioassay (Recombinants), Immunology, Antibodies


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Product FAQs

 
How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
For a complete list of FAQs on all our products, visit our FAQ page.
 
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Related Pathways

Pathway
Chemokine Receptor Biology
Immunologic Networks 2011
Innate Immunity


Related Categories

Category
Cell Biology > Growth Factors/Growth Factor Receptors > CCL2
Cytokines & Chemokines > Human Cytokine & Chemokine Research > Recombinant Proteins > CCL2 (MCP-1)
Cytokines & Chemokines > Recombinant Proteins > Human > CCL2 (MCP-1)

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Version: 1 Revision Date: 2012-11-30
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