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Recombinant Human IL-8 (carrier-free)
Recombinant Human IL-8 (carrier-free)
574202
10 µg
¥25,000
574204
25 µg
¥45,000
574206
100 µg
¥116,000
574208
500 µg
¥280,000
Product Details
Source:
Human IL-8, amino acids Ser28-Ser99 (Accession # NM_000584) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in PBS
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration:
The 10, 25, and 100 µg sizes are bottled at a concentration of 200 µg/mL. The 500 µg size is bottled at bulk concentration (see vial or CoA).
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
Bioactivity was measured by its property to chemoattract human peripheral blood neutrophils in a dose dependent manner. The highest chemotactic activity was detected from 10 to 100 ng/ml.
IL-8 (CXCL8) was originally recognized as a neutrophil-activating protein on the basis of two in-vitro effects, chemotaxis and the release of granule enzyme. IL-8 is generated as a precursor of 99 amino acids. Once the mature cytokine is secreted, the amino-terminal is processed to yield several biologically active variants. The predominant variant consists of 72 amino acids. IL-8 is one of the ELR (Glu-Leu-Arg) motif-positive CXC-chemokines and shares similarities with angiogenic cytokines.
Other Names:
Small inducible cytokine subfamily B, member 8 (SCYB8), Monocyte derived neutrophils chemotactic factor (MDNCF), Neutrophil-activating peptide 1 (NAP1), Granulocyte chemotactic protein 1 (GCP1), Chemochine, CXC motif, ligand 8, CXCL8
Structure:
Chemokine
Distribution:
IL-8 is expressed by neutrophils, mococytes, macrophages, mast cells, endothelial cells, fibroblasts, keratinocytes, synovial cells, chondrocytes, epithelial cells, megakariocytes, melanocytes, hepatocytes, mesangial, and tumor cells. Most primary and metastatic solid tumors, such as breast, uterine, prostate, colon, and pancreatic carcinomas, melanoma, and glioblastoma, constitutively express IL-8.
Function:
CXCL8 has diverse functions in immune surveillance, inflammation, and angiogenesis. CXCL8 directly modulates endothelial cell proliferation and migration, thus promoting angiogenesis. IL-8 modulates myelopoiesis by suppressing the myeloid progenitor proliferation. IL-8 also modulates the homing of hematopoitic progenitors by affecting the secretion of MMPs and TIMPs from bone marrow and peripheral blood CD34+ cells. Monocytes and macrophages generate IL-8 upon stimulation with IL-1α, IL-1β, TNFα, IL-3, GMCSF, endotoxin, lectins, phorbol esters, immune complexes, and phagocytosis. Opsonized particles are also a prime stimulus for IL-8 production by neutrophils.
Ligand Receptor:
CXCR1 (CDw128a) and CXCR2
Interaction:
Neutrophils, basofils, highly cytotoxic CD8 T cells, megakariocytes, endothelial cells.
Antigen References:
1. Hess C, et al. 2004. Blood 104:3463. 2. Emadi S, et al. 2005. Blood 105:464. 3. Matsuo Y, et al. 2009. Int. J. Cancer 4:853. 4. Drewniak A, et al. 2010. Blood 115:4588.
Recombinant Human IL-8 (carrier-free), Recombinant, Small inducible cytokine subfamily B, member 8 (SCYB8), Monocyte derived neutrophils chemotactic factor (MDNCF), Neutrophil-activating peptide 1 (NAP1), Granulocyte chemotactic protein 1 (GCP1), Chemochine, CXC motif, ligand 8, CXCL8, Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
