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Recombinant Human IL-5
Recombinant Human IL-5
560701
2 µg
$125.00
Product Details
Source:
E. coli, approximately 26 kD
Formulation:
Sterile-filtered through a 0.2 micron filter. Lyophilized with no additives.
Preparation:
Centrifuge vial prior to opening. Reconstitute in 100 µl sterile water for a concentration of 20 µg/ml and mix well. This solution can be diluted into other aqueous buffers containing a carrier protein such as 1% BSA or HSA or 10% FBS. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer with carrier protein. After reconstitution, the cytokine can be stored at -20°C to -70°C for up to three months.
Storage & Handling:
The lyophilized protein is stable for 12 months from the date of receipt at -20°C to -70°C. Reconstituted hIL-5 is stable for three months when stored in working aliquots, with a carrier protein, at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. Please see antibody product data sheets and recommended protocols. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA: This IL-5 protein is useful as a standard for a human IL-5 sandwich ELISA, using unlabeled JES1-39D10 antibody (catalog #500902) for capture and biotinylated JES1-5A10 antibody (catalog #501002) for detection. Ligand Blocking: This IL-5 protein is useful as a ligand-blocking specificity control for immunohistochemical or immunofluorescent staining. Bioassay: This IL-5 protein is biologically active, and can be used for in vitro assays.
Description:
IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes.
Other Names:
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T cell replacing factor-1 (TRF-1)
Structure:
Cytokine; dimer
Regulation:
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Cellular Sources:
Mast cells, T cells, bone marrow stromal cells
Cellular Targets:
Eosinophils
Receptors:
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Bioactivity/Activities:
Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Antigen References:
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107. 3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299. 4. Takatsu, K. 1991. Microbiol. Immun. 35:593.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
