Sterile-filtered solution comprised of PBS, pH 7.2, with FBS as a carrier and stabilizer.
Storage & Handling:
After thawing, quick spin vial. Make working aliquots for storage at ≤ -70°C to minimize repeated freeze/thaw cycles. Any further dilution of the cytokine should be done using a buffered diluent containing a protein carrier (e.g., 1% BSA or 10% FBS). For greatest stability, the cytokine protein is best kept at relatively high concentrations and in the presence of a protein carrier.
Application:
ELISA, Ligand Block, Bioassay
Recommended Usage:
Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 500 pg/ml to 4 pg/ml is suggested. Please see antibody product data sheets and recommended protocols. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
ELISA: This IL-5 protein is useful as a standard for a human IL-5 sandwich ELISA, using unlabeled JES1-39D10 antibody (catalog #500902) for capture and biotinylated JES1-5A10 antibody (catalog #501002) for detection. Ligand Blocking: This IL-5 protein is useful as a ligand-blocking specificity control for immunohistochemical or immunofluorescent staining. Bioassay: This IL-5 protein is biologically active, and can be used for in vitro assays.
Description:
IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes.
Other Names:
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T cell replacing factor-1 (TRF-1)
Structure:
Cytokine; dimer
Regulation:
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Cellular Sources:
Mast cells, T cells, bone marrow stromal cells
Cellular Targets:
Eosinophils
Receptors:
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Bioactivity/Activities:
Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Antigen References:
1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Takatsu, K., et al., 1988. Immunol. Rev. 102:107. 3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299. 4. Takatsu, K. 1991. Microbiol. Immun. 35:593.
