Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Recombinant Human IL-4 (carrier-free)
Recombinant Human IL-4 (carrier-free)
574002
10 µg
¥25,000
574004
25 µg
¥45,000
574006
100 µg
¥135,000
574008
500 µg
¥360,000
Product Details
Source:
Human IL-4, amino acids His25-Ser153 (Accession# NM_000589) was expressed in E.coli.
Formulation:
The protein was 0.22 µm filtered in 10mM NaH2PO4, 150mM NaCl, pH7.2.
Endotoxin Level:
Less than 0.01ng per µg cytokine as determined by the LAL method
Preparation:
For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer containing carrier protein such as 1% BSA or HSA or 10% FBS.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. After dilution, the cytokine could be storage at 4°C for one month and up to 3 months from -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity:
ED50=0.2 - 0.6 ng/ml, corresponding to a specific activity of 1.65 - 5 x 106 units/mg, as determined by the dose dependent stimulation of TF-1 cell proliferation.
IL-4 is the primary cytokine implicated in the development of Th2-mediated responses, which is associated with allergy and asthma. The Type I receptor comprises IL-4Rα and the common gamma-chain (γc), which is also shared by the cytokines IL-2, -7, -9, -15 and -21 and is present in hematopoietic cells. IL-4 can use the type II complex, comprising IL-4Rα and IL-13Rα1, which is present in non-hematopoietic cells. This second receptor complex is a functional receptor for IL-13, which shares approximately 25% homology with IL-4. The type I receptor complex can be formed only by IL-4 and is active in Th2 development. In contrast, the type II receptor complex formed by either IL-4 or IL-13 is most active during airway hypersensitivity and mucus secretion and is not found in T cells.
IL-4 is produced by Th2 cells, naive CD4+ T cells, NKT cells, and basophils.
Function:
IL-4 has a crucial role in the differentiation of TH2 cells and induction of Th2 associated cytokines. IL-4, through its activation of STAT6, upregulates GATA3 expression and also suppresses TH1 and TH17 cell responses, partly through the upregulation of growth factor independent 1(GFI1), a transcriptional repressor of IFNγ and IL-17 production. IL-4 induces macrophage activation and TSLP production. IL-4 recruits and activates IgE-producing B cells (IgE class switching) and enhances IgE-mediated responses by up-regulating IgE receptors on B lymphocytes, mast cells, and basophils. In addition, IL-4 also induces VCAM-1 on vascular endothelium and thus directs the migration of T lymphocytes, monocytes, basophils, and eosinophils to the inflammation site.
Ligand Receptor:
IL-4 signals through Type I (IL-4Rα, γc) and Type II receptors (IL-4Rα, IL-13Rα1) complexes.
Interaction:
T cells, B cells, macrophages, epithelial cells, smooth muscle cells, and bronchial fibroblasts.
Antigen References:
1. Swain SL, et al. 1990. J. Immunol. 145:3796. 2. Hsieh CS, et al. 1992. P. Natl. Acad. Sci. USA 89:6065. 3. Allison-Lynn A, et al. 2006. J. Immunol. 176:7456. 4. Kato A, et al. 2007. J. Immunol. 179:1080. 5. LaPorte SL, et al. 2008. Cell 132:259. 6. Martinez FO, et al. 2009. Annu. Rev. Immunol. 27:451.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
