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Recombinant Human IL-3 (carrier-free)
Recombinant Human IL-3 (carrier-free)
578002
10 µg
¥30,000
578004
25 µg
¥59,000
578006
100 µg
¥99,000
578008
500 µg
¥235,000
Product Details
Source:
Human IL-3, amino acids Ala20-Phe152 (Accession# P08700) was expressed in Insect cells.
Formulation:
0.22 µm filtered protein solution is in 20 mM NaH2PO4, 0.30 M NaCl, pH 6.0.
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
10-100 µg sizes are bottled at 200 µg/mL; 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at -20°C or -70°C for six months. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
ED50 = 0.10 - 0.30 ng/ml, corresponding to a specific activity of 0.33 - 1.0 x 107 units/mg, as determined by the dose dependent stimulation of TF-1 cell proliferation assay. Under this assay the bioactivity is equivalent to competit
Interleukin-3 is a hemopoietic growth factor involved in the survival, proliferation, and differentiation of multipotent hemopoietic cells. IL-3 is the most potent growth factor for basophils, followed by granulocyte-macrophage colony-stimulating factor and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and releasability. IL-3 is highly expressed by mast cells, and large and rapidly released amounts of autocrine IL-3 production is responsible for mast cell survival by IgE in the absence of antigen.IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma, atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis. IL-3 stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. IL-3 plays a vital role in stimulating basophils and mast cell responses to parasite infections, and since basophils play an important role in Th2 immune responses, IL-3 is a critical regulator of allergic inflammation. IL-3 is expressed in the major embryonic vessels and regulates the survival and proliferation of hematopoietic stem cells in the early stages of embryonic development.
Activated T cells, Th1, Th2, mast cells, eosinophils, keratinocytes, NK cells, and endothelial cells.
Function:
IL-3 is a pleiotropic factor which promotes both self-renewal and differentiation of early multipotential progenitors and is involved in inducible hematopoiesis in response to infections.
Ligand Receptor:
Heterodimer IL-3Rα (CD123) a common β subunit (hβc) that is shared with IL-5R/GM-CSFR.
1. Kitamura T, et al. 1989. J. Cell. Physiol. 140:323. 2. Kohno M, et al. 2005. Blood 105:2059. 3. Murphy JM and Young IG, 2006. Vitam. Horm. 74:1. 4. Kleemann R, et al. 2008. Cardiovasc. Res. 79:360. 5. Valent P, and Dahinden CA. 2010. Curr. Opin. Hematol. 17:60. 6. Mirza S, et al. 2010. J. Biol. Chem. 285:22370.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
