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Recombinant Human IL-22 (carrier-free)
Recombinant Human IL-22 (carrier-free)
571302
10 µg
$195.00
571304
25 µg
$395.00
571306
100 µg
$900.00
571308
500 µg
$2,500.00
Product Details
Source:
Human IL-22, amino acids Ala34-Ile179 (Accession # NM_020525) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH7.2.
Endotoxin Level:
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Preparation:
10-100µg sizes are bottled at 200µg/mL. 500µg sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months or -20°C to -70°C for six months. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity:
The ED50 is 0.062-0.177 ng/ml, corresponding to a specific activity of 1.61-0.56 X107 units/mg, as determined by a dose dependent stimulation of human Colo205 cells in production of IL-10.
Use when high specific biological activity is required.
COA:
Application Notes:
Ligand Blocking: This IL-22 protein is useful as a ligand-blocking specificity control for immunohistochemical or immunofluorescent staining. Bioassay: This IL-22 protein is biologically active, and can be used for in vitro assays.
Description:
IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells.IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely Il-10, IL-19, IL-20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79% amino acid identity with mouse IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4 (+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases.
thymic lymphomas, T cells, activated T cells, macrophages, and mast cells
Function:
Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
Ligand Receptor:
IL-22R1 and IL-10R2 receptor chains
Interaction:
Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
Bioactivity/Activities:
IL-22 bioactivity was measured by its ability to induce IL-10 in human Colo205 cells
Antigen References:
1. Nagalakshmi ML, et al. Intern Immunopharmacol 4:679-691 2004. 2. Kebir H, et al.Nat. Med. 13:1173-1175 2007. 3. Gu Y, et al.Eur. J. Immunol. 38:1807-1813 2008. 4. Pene J, et al.J. Immunol. 180:7423-7430 2008. 5. Dumotier L, et al.J immune 164:1814-1819 2000. 6. Xie MH, et al.J. Biol. Chem. 765:31335-31339 2000. 7. Jones BC, et al.Structure July 2 2008. 8. Kotenko SV, et al.J. Immunol. 166:7096-71033 2001. 9. Chang C, et al.Cell Res 16:902-907 2006.
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