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Recombinant Human IL-2 (carrier-free)
Recombinant Human IL-2 (carrier-free)
589102
10 µg
¥25,000
589104
25 µg
¥45,000
589106
100 µg
¥119,000
589108
500 µg
¥380,000
Product Details
Source:
Human IL-2, amino acids Ala21-Thr153 (Accession # NM_000586), was expressed in insect cells.
Formulation:
The protein was 0.22 µm filtered protein solution is in 10mM NaH2PO4, pH 7.2, 150mM NaCl.
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
10-100 µg sizes are bottled at 200 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
ED50 =0.175 - 0.8 ng/ml, corresponding to a specific activity of 1.25 -5.71 x106 units/mg, as determined by the dose dependent stimulation of CTLL2 cell proliferation.
This IL-2 protein is biologically active and can be used for in vitro assays.
Application References:
1. Malu DT, et al. 2011. J. Immunol. 186:6271. PubMed.
CTLL-2 cell proliferation induced by human IL-2.
Description:
IL-2 was discovered through its function as a T cell growth factor (TCGF), and plays a pivotal role in immune responses against pathogenic infection. Recognition and binding of the foreign Ags by the TCRs stimulate both the secretion of IL-2 and the expression of IL-2Rs on the T cell surface. Subsequently, the IL-2/IL-2R interaction activates the intracellular Ras/Raf/MAPK, JAK/STAT, and PI3K/AKT signal pathways, and ultimately stimulates the growth, differentiation, and survival of the Ag-selected cytotoxic T cells. Human IL-2 acts on murine and human T cells, and its receptors are shared by others cytokines. IL-2Ra is an IL-2–specific receptor, IL-2Rb is shared with IL-15 and the gc is a common receptor shared by many cytokines including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21.
IL-2 works in vivo to promote clonal T-cell expansion during immune responses. IL-2 stimulates the growth, differentiation, and survival of the Ag-selected cytotoxic T lymphocytes (CTLs). In addition, IL-2 regulates facilitate the proliferation and the synthesis of immunoglobulin by B cells, induces the generation and persistence of natural killer (NK) cells. Also, IL-2, through its role in activation-induced cell death (AICD) and its participation in the maintenance of peripheral CD4+CD25+ regulatory T (TReg) cells, is involved in the elimination of self-reactive T cells, which have a role in the pathogenesis of autoimmune diseases.
Ligand Receptor:
IL-2R is composed of three subunits, IL-2R alpha (p55, Tac Ag, or CD25), IL-2R beta (p75 or CD122), and the γc chain (p65 or CD132).
Interaction:
T cells, B cells, NK cells, LAK cells, monocytes, macrophages, oligodendrocytes
Antigen References:
1. Smith KA. 1988. Science 240:1169. 2. D’Cruz LM, et al. 2005. Nature Immunol. 6:1152. 3. Maloy KJ, et al. 2005. Nature Immunol. 6:1071. 4. Waldmann TA. 2006. Nature Rev. Immunol. 6:595. 5. Ma A, et al. 2006. Annu. Rev. Immunol. 24:657. 6. Du J, et al. 2010. J. Immunol. 184:1361.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
