Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Recombinant Human IL-1α (carrier-free)
Recombinant Human IL-1α (carrier-free)
570002
10 µg
¥39,000
570004
25 µg
¥79,000
570006
100 µg
¥180,000
570008
500 µg
¥420,000
Product Details
Source:
Human IL-1α, amino acids Ser113-Ala271 (Accession# NM_000575) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
Endotoxin Level:
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Preparation:
10-100µg sizes are bottled at 200µg/ml. 500µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity:
The ED50 is 0.58 - 1.45 pg/ml, corresponding to a specific activity of 1.72-0.689 x 109 units/mg, as determined by the dose dependent stimulation of D10 cells proliferation.
Use when high specific biological activity is required.
COA:
Application Notes:
This IL-1α protein is biologically active, and can be used for in vitro assays
Description:
IL-1 was isolated from human blood that had been exposed to a pathogenic bacterium. IL-1 is a pyrogen, and it is an activating factor for lymphocytes. It also damaged joints and influenced liver proteins (2). IL-1α binds to the cell surface type I and II IL-1 receptors (IL-1RI and IL-1RII). IL-1 and -β and IL-1RA can compete for binding to these receptors. However, only IL-1RI, not IL-1RII, is functional because IL-1RII lacks a cytoplasmic domain and is thus unable to transmit signals to downstream steps (3). IL-1α is expressed by cancer cells and promotes angiogenesis and metastasis of pancreatic cancer and human gastric cancer cell lines (4). In addition, IL-1 induced the expression of VEGF in colon cancer (5).
Other Names:
interleukin 1, alpha, IL-1A, IL1F1
Structure:
Cytokine
Distribution:
IL-1α is produced by monocytes, macrophages, keratinocytes, stimulated B lymphocytes, fibroblasts, endothelial cells, and epithelial cells.
Function:
The cytokine IL-1α is a key molecule both in the innate and in the adaptive immune response. IL-1α induces autonomous proliferation in vascular smooth muscle cells, T cell activation during Ag presentation, up-regulation of monocyte/macrophage-mediated tumor cytotoxicity, stimulation of osteoclast formation, and production of proteinases in huma chondrocytes (1).
Ligand Receptor:
Type I and II IL-1 receptors (IL-1RI and IL-1RII)
Antigen References:
1. Yatabe T, et al. Ann Rheum Dis published online 28 Jul 2008. 2. Ledford H Nature 450:29-30 2007. 3. Boraschi B Tagliabue A Vitam Horm 74:229-254 2006. 4. Shao J and Sheng H J. Immunol. 178:4097-4103 2007. 5. Elaraj DM, et al.Clin Cancer Res 12:1088-1096 2006.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
