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Recombinant Human IL-17A/IL-17F Heterodimer (carrier-free)
Recombinant Human IL-17A/IL-17F Heterodimer (carrier-free)
580602 10 µg $285.00     
580604 25 µg $385.00     
580606 100 µg $1,350.00     
580608 500 µg $5,600.00     
Product Details
Formulation: 0.22 µm filtered protein solution is in 10 mM NaH2PO4, 300 mM NaCl, pH 7.2.
Endotoxin Level: Endotoxin level is < 0.1 EU/µg (< 0.01 ng/µg) protein as determined by the LAL method.
Preparation: 10-100 µg sizes are bottled at 200 µg/mL. 500 µg sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling: Unopened vial can be stored at 4°C for three months or -20° to -70°C for six months. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term-storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity: Activity was tested by induction of IL-6 in human skin fibroblasts by IL-17A/F. The ED50 is 15-25 ng/ml, corresponding to a specific activity of 6.6 - 4 x 104 units/mg. Under this assay the activity was comparable to other vendor's cytokine.
Application:

Bioassay
COA:
Enter Lot#:   
 Induction of IL-6 in human
Induction of IL-6 in human skin fibroblast by IL-17A/IL-17F heterodimer.


Description:

IL-17A/F is part of the IL-17 cytokine family which consists of six structurally related proteins (IL-17A, B, C, D, E, and F). IL-17A is expressed primarily by Th17 cells, a subset of CD4 T cells. IL-17F is most closely related to IL-17A. The two molecules share 50% aminoacid sequence homology. Like IL-17A, IL-17F mRNA and protein have been detected in Th17 cells. IL-17F and IL-17A exist as a homodimers, adopting a cysteine knot motif formed through the interactions of four cysteines, one of which is responsible for the interchain bonding. IL-17F and IL-17A can form both homodimeric and heterodimeric proteins when expressed in a recombinant system and all forms of the recombinant proteins have in vitro functional activity. In addition, activated human CD4-T cells produce the homodimers of IL-17F, IL-17A, and the IL-17F/IL-17A heterodimer.
Other Names: IL-17A/F
Distribution: IL-17A/F is expressed by Th17 cells and activated CD4 T cells
Function: IL-17A/F induces the expression of CXCL1/GROα in a human bronchial epithelial cell line and primary human skin fibroblasts
Ligand Receptor: IL-17A/F binds to IL-17RA and IL-17RC
Interaction: IL-17A/F might act primarily on parenchymal cells such as fibroblasts, epithelial cells, and endothelial cells
Cellular Sources: Human IL-17A, amino acids Ile20-Ala155 (Accession # NM_002190) and human IL-17F, amino acids Arg31-Gln163 (Accession # AF384857) were expressed independently in E. coli.
Antigen
References:

1. Chang SH, et al. Cell Res 17:435-440 2007.
2. Liang SC, et al. JI 179:7791-7799 2007.
3. Wright JF, et al. J. Biol. Chem. 282:13447-13455 2007.
4. Wright JF, et al. J. Immunol. 181:2799-2805 2008.
5. Fouser LA, et al. Immunol. Rev. 226:87-102 2008.
GeneID: 112744
Latest Publications: View the latest IL-17A/IL-17F articles on HighwirePress.com
UniProt: View information about IL-17A/IL-17F on UniProt.org
Keywords: Recombinant Human IL-17A/IL-17F Heterodimer (carrier-free), Recombinant, IL-17A/F, Immunology, Antibodies
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