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Recombinant Human IFN-γ (carrier-free)
Recombinant Human IFN-γ (carrier-free)
570202
10 µg
¥14,000
570204
25 µg
¥25,000
570206
100 µg
¥39,000
570208
500 µg
¥120,000
Product Details
Source:
Human IFN-γ, amino acids Gln24-Gln166 (Accession # NM_000619) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in 20mM NaH2PO4,150mM NaCl, pH 7.2.
Endotoxin Level:
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Preparation:
10-100µg sizes are bottled at 100µg/ml. 500µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months or from -20°C to -70°C for six months. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/ml in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
The ED50 is 0.416 ng/ml, corresponding to a specific activity of 2.4 X106 units/mg, as determined by a dose dependent stimulation of HT-29 cells.
Use when high specific biological activity is required.
COA:
Description:
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Other Names:
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF),
Structure:
Cytokine; dimer
Regulation:
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Cellular Sources:
CD8+ and CD4+ T cells, NK cells
Cellular Targets:
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors:
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity/Activities:
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321. 3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571. 4. Gray P, et al. 1987. Lymphokines 13:151.
Recombinant Human IFN-γ (carrier-free), Recombinant, Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), , Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
