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Recombinant Human G-CSF
Recombinant Human G-CSF
561701
2 µg
¥25,000
Product Details
Source:
E. coli, approximately 18.7 kD
Formulation:
Sterile-filtered through a 0.2 micron filter. Lyophilized with no additives.
Preparation:
Centrifuge vial prior to opening. Reconstitute in 100 µl sterile water for a concentration of 20 µg/ml and mix well. This solution can be diluted into other aqueous buffers that containing a carrier protein such as 1% BSA or HSA or 10% FBS. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer with carrier protein. After reconstitution, the cytokine can be stored at -20°C to -70°C for up to 3 months.
Storage & Handling:
The lyophilized protein is stable for 12 months from the date of receipt at -20°C or -70°C. Reconstituted G-CSF is stable for at least three months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.
Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 4000 pg/ml to 30 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA: This G-CSF protein is useful as a standard for a human G-CSF sandwich ELISA, using unlabelled BVD13-3A5 antibody (catalog #502102) for capture and biotinylated BVD11-37G10 antibody (catalog #506702) for detection. Ligand Blocking: This G-CSF protein is useful as a ligand-blocking specificity control for immunohistochemical or immunofluorescent staining. Bioassay: This G-CSF protein is biologically active, and can be used for in vitro assays.
Description:
Granulocyte - colony stimulating factor (G-CSF) is a potent stimulator of bone marrow cells especially those of neutrophilic granulocyte lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IFN-gamma. Human G-CSF is an 18.7 kD protein containing 174 amino acids.
Upregulated by IL-1, IL-17, TNF-α, IFN-γ, GM-CSF; downregulated by prostaglandin E2
Cellular Sources:
Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, bone marrow stromal cells
Cellular Targets:
Hematopoietic progenitors of neutrophil/granulocyte lineage, endothelial cells
Receptors:
CD114 (G-CSFR)
Bioactivity/Activities:
Growth/differentiation/activating factor for neutrophils; proliferation/migration endothelial cells; synergistic with IL-3 growth of hematopoietic progenitors
Antigen References:
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Demetri, G., et al. 1991. Blood 78:2791. 3. Moore, M. 1991. Annu. Rev. Immunol. 9:159. 4. Negrin, R., et al. 1992. Adv. Pharmacol.. 23:263.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
