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Recombinant Human FGF-basic/145aa (carrier-free)
Recombinant Human FGF-basic/145aa (carrier-free)
571502
10 µg
¥25,000
571504
25 µg
¥45,000
571506
100 µg
¥110,000
571508
500 µg
¥320,000
Product Details
Source:
Human FGF-basic, amino acids Ala144-Ser288 (Accession # NM_002006) was expressed in E. coli.
Reactivity:
Human
Formulation:
0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2, 1mM DTT.
Endotoxin Level:
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Preparation:
10-100µg sizes are bottled at 200µg/mL. 500µg sizes and larger are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at -20°C for six months or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. For long term storage, aliquot into polypropylene vials and store in a manual defrost freezer. Avoid repeated freeze/thaw cycles.
Activity:
ED50 = 1 - 4 ng/ml, corresponding to a specific activity of 1 - 0.25 x 106 units/mg, as determined by the dose dependent stimulation of NIH/ 3T3 cell proliferation. The bioactivity is equivalent to competitor reported values.
3NIH/3T3 cell proliferation induced by human FGFb.
Description:
FGFb is a member of the fibroblast growth factor (FGF) family which includes 23 members. FGFb is expressed in almost all tissues and play important roles in a variety of normal and pathological processes, including development, wound healing and neoplastic transformation. FGFb is mitogenic for many cell types, both epithelial and mesenchymal. FGFb shows potent angiogenic activity and has been implicated in tumor angiogenesis (2). In prostate, bladder, and renal cancers, FGFb regulates the induction of metalloproteinases (MMP) that degrade extracellular matrix proteins, thus facilitating tumor metastasis (3). FGFb binds to a family of four distinct, high affinity tyrosine kinase receptors, designated FGFR-1 to -4 (4). In addition, FGFb binds to the ECM, and heparan sulfate (HS) is an essential and dynamic regulator of fibroblast growth factor (FGF) signaling. Two fundamentally different crystallographic models have been proposed to explain, at the molecular level, how HS/heparin enables FGF and FGF receptor (FGFR) to assemble into a functional dimer on the cell surface (5), although there is controversy regarding the exact manner by which this occurs.
FGFb is a potent angiogenic factor, and plays a key role in various physiological and pathological conditions, including embryonic development, wound repair, inflammation, and tumor growth (1).
Ligand Receptor:
FGFR-1 (flg), FGFR-2 (bek, K-sam), FGFR3, and FGFR-4 (flg-2); low affinity coreceptor heparin sulfate and heparin sulfate proteoglycans required for full activity.
FGFb is a heparin-binding growth factor that is an angiogenic agent in vivo and also a potent mitogen for a variety of cell types in vitro.
Antigen References:
1. Rusnati M and Presta M Current Pharm Des 13:2025-2044 2007. 2. Chaffer CL, et al.Differentiation 75(9):831-42 2007. 3. Cronauer NV, et al.Eur Urol 43:309-319 2003. 4. Shimizu A, et al.J. Biol. Chem. 276:11031-11040 2001. 5. Mahammadi M, et al.Curr Opin Struct Biol 15:506-516 2005.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
