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Recombinant Human CXCL5 (ENA-78) (carrier-free)
Recombinant Human CXCL5 (ENA-78) (carrier-free)
573402
10 µg
¥25,000
573404
25 µg
¥45,000
573406
100 µg
¥119,000
573408
500 µg
¥480,000
Product Details
Source:
Human CXCL5, amino acids Ala37-Asn114 (Accession# NM_002994.3) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in PBS.
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
10-100 µg sizes are bottled at 200 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
Bioactivity was measured by its property to chemoattract human neutrophils in a dose dependent manner.
CXCL5 is an ELR+CXC chemokine characterized by a Glu-Leu-Arg (ELR) motif preceding the characteristic CXC sequence. CXCL5 chemoattracts and activates neutrophils during inflammation through the binding to CXCR2. CXCL5 occurs in different NH2- and COOH-truncated forms, and both NH2-terminal and COOH-terminal truncation result in an increase in neutrophil chemotactic potency. CXCL5(8–78) and CXCL5(9–78) induce a higher neutrophil influx in vivo compared with CXCL5(1–78). CXCL5 regulates the availability of binding sites for other ELR+ CXC chemokines released during inflammation, through its interaction with erythrocyte duffy antigen receptor (DARC). As a result, CXCL5 induces the increase of CXCL1 and CXCL2 in a model of pulmonary inflammation. Therefore, CXCL5 acts as a regulator of chemokine scavenging and pulmonary host defense to bacterial infection. Lung epithelial cells express CXCR2; consequently, CXCL5 may modulate neutrophil transepithelial and transendothelial migration. High levels of CXCL5 have been associated to prostate tumor, gastric cancer, and pancreatic cancer progression.
Endothelial cells, fibroblasts, neutrophils, monocytes, macrophages, platelets, alveolar type II cells
Function:
CXCL5 chemoattracts and activates neutrophils during inflammation, and it is induced by LPS and inflammatory cytokines. NH2-terminal truncation enhances, whereas citrullination decreases the chemotactic properties of CXCL5.
Ligand Receptor:
CXCR2
Interaction:
Neutrophils
Antigen References:
1. Walz A, et al. 1991. J. Exp. Med. 174:1355. 2. Wuyts A, et al. 1999. J. Immunol. 163:6155. 3. Jeyaseelan S, et al. 2005. Am. J. Respir. Cell. Mol. Biol. 32:531. 4. Mei J, et al. 2010. Immunity 33:106. 5. Mortier A, et al. 2010. J. Biol. Chem. 285:29750. 6. Grommes J and Soehnlein O. 2011. Mol. Med. 17:293. 7. Liu Y, et al. 2011. J. Immunol. 186:3197. 8. Kuo PL, et al. 2011. J. Cell. Physiol. 226:1224.
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