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Recombinant Human CXCL13 (carrier-free)
Recombinant Human CXCL13 (carrier-free)
574702
10 µg
¥21,000
574704
25 µg
¥39,000
574706
100 µg
¥119,000
574708
500 µg
¥350,000
Product Details
Source:
Human CXCL13, amino acids Val23-Arg94 (Accession# NM_006419.2) was expressed in E. coli.
Formulation:
0.22 µm filtered protein solution is in PBS.
Endotoxin Level:
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Preparation:
10-100 µg sizes are bottled at 200 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 10% FBS. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity:
Bioactivity was measured by its property to chemoattract Baf3-hCXCR5 transfectants in a dose dependent manner.
Human CXCR5 transfectants chemoattracted by human CXCL13.
Description:
CXCL13 was initially described as a chemokine highly expressed in the follicles of Peyer's patches, spleen, and lymph nodes and as a strong chomoattractant for B cells. CXCL13 is expressed by T follicular helper cells, dendritic cells, and stromal cells in B cell areas of secondary lymphoid tissue. CXCR5, the receptor for CXCL13, was initially cloned from Burkitt’s lymphoma cells and named BLR1. CXCR5 is highly expressed in those cells and B cells. CXCL13, CCL19, and CCL21 are involved in the formation of tertiary lymphoid tissues in chronic antigen-induced arthritis. CXCL13 takes part in the allergic airway inflammatory process. In addition, high expression of CXCL13 has been linked to lupus, Helicobacter pylori-associated gastritis, multiple sclerosis, and rheumatoid arthritis. In summary, CXCL13 is highly expressed at the sites of new lymphoid tissue formation in a variety of chronic inflammatory conditions.
Other Names:
B cell attracting chemokine (BCA-1), B- lymphocyte chemoattractant (BLC)
Structure:
Chemokine
Distribution:
Follicular dendritic cells, monocytes, macrophages, and stromal cells from germinal centers. CXCL13 is derived from endothelial cells, synovial fibroblasts, and follicular dendritic cells in RA.
Function:
CXCL13 chemoattracts B cells and is a key chemokine for B-cell entry into lymphoid follicles. It also takes part in the formation of ectopic lymphoid tissue in chronic inflammation. CXCL13 production by follicular Th cells is induced by TCR activation.
Ligand Receptor:
CXCR5 (CD185).
Interaction:
B cells and follicular helper CD4 T cells.
Antigen References:
1. Gunn MD, et al. 1998. Nature 391:799. 2. Legler DF, et al. 1998. J. Exp. Med. 187:655. 3. Kim CH, et al. 2004. Blood 104:1952. 4. McDonald KG, et al. 2010. Am. J. Pathol. 176:2367. 5. Gatto D, et al. 2011. J. Immunol. 187:4621.
Recombinant Human CXCL13 (carrier-free), Recombinant, B cell attracting chemokine (BCA-1), B- lymphocyte chemoattractant (BLC), Immunology, Antibodies
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Clone
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