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Human CCL21, amino acids Ser24-Pro134 (Accession# NM_002989.2) was expressed in E. coli.
The 111 amino acid recombinant protein has a predicted molecular mass of approximately 12 kD. The DTT-reduced protein migrates at approximately 16 kD and non-reduced protein at approximately 17 kD by SDS-PAGE. The N-terminal amino acid is Ser.
>98%, as determined by Coomassie stained SDS-PAGE.
0.22 µm filtered protein solution is in PBS.
Less than 0.01 ng per µg cytokine as determined by the LAL method.
10-100 µg sizes are bottled at 200 µg/mL. 500 µg and larger sizes are bottled at the concentration indicated on the vial.
Storage & Handling:
Unopened vial can be stored at 4°C for three months, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1% BSA or HSA. After dilution, the cytokine can be stored at 4°C for one month or from -20°C to -70°C for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity was measured by its property to chemoattract Baf3-hCCR7 transfectants in a dose dependent manner.
Baf3-hCCR7 transfectants chemoattracted by human CCL21.
CCL21 is a CC chemokine highly expressed in lymph nodes. CCL21 is only 32% identical to CCL19 at the amino acid level; nevertheless, these chemokines bind to the same receptor CCR7. CCL21 has a unique extension of about 30 amino acids with two extra cysteine residues in its carboxyl terminus. CCL21 is expressed in synovial tissue in rhematoid arthritis (RA), and it has been suggested that CCL21, but not CCL19, induces human microvascular endothelial cell (HMVEC) migration and/or tube formation. In addition, it has been reported that sustained high concentrations of either soluble or immobilized CCL19 and CCL21 inhibit T cell proliferation and IL-2 secretion.
6CKine; Secondary lymphoid tissue chemokine, SLC; Exudos 2; Small inducible cytokine subfamily A, member 21 (SCYA21)
CCL21 is highly expressed in HEV of lymph nodes and Peyer’s patches. Also, it is expressed in the T cell zones of lymph nodes, in Peyer’s patches by reticular cells, and in spleen by stromal cells. CCL21 is expressed by endothelial cells in lymphatic venules.
CCL21 chemoattracts naïve T cells and maturing dendritic cells (DCs) into T zones of secondary lymphoid organs. CCL21 and CCL19 chemoattract antigen-engaged B cells to the boundary of B and T zones to interact with T-helper cells. CCL19 and CCL21 inhibit apoptosis in DCs.
Thymocytes, activated T cells, naïve CD4 and CD8, mature DCs, fibroblast-like synoviocytes of patients with rheumatoid arthritis (RA).
1. Hedrick JA and Zlotnik A. 1997. J. Immunol. 159:1589. 2. Reif K, et al. 2002. Nature 416:94. 3. Ziegler E, et al. 2007. J. Immunol. 179:6485. 4. Pickens SR, et al. 2012. Arthritis Rheum. 10.1002.
Recombinant Human CCL21 (6CKine) (carrier-free), Recombinant, CCL21 recombinant protein, recombinant CCL21 protein, 6CKine; Secondary lymphoid tissue chemokine, SLC; Exudos 2; Small inducible cytokine subfamily A, member 21 (SCYA21), Bioassay (Recombinants), Immunology, Antibodies
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