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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
COA:
Application Notes:
The RTK4174 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofleorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 400710) as negative control.
Application References:
1. Ganeshah K, et al. 2012. J. Immunol. 188:594. PubMed. 2. D'Agostino PM, et al. 2012. PNAS. 109:6175. PubMed.
Description:
The isotype of RTK4174 immunoglobulin is rat IgG2c,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Purified Rat IgG2c, κ Isotype Ctrl, RTK4174, Purified, Immunology, Antibodies
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