Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes:
The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofleorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 400622) as negative control.
Application References:
1. Cervantes-Barragan, L., et al., 2007. Blood 109:1131. 2. Zeiser, R., et al., 2007. Blood 109:2225. 3. Sasaki K. et all. 2008. J.Immunol.181:104.PubMed 4. Duan, J., et al.2008 Proc Natl Acad Sci U S A. 105:5183.PubMed 5. Yi, H., et al. 2009. Blood. 113:5819. PubMed 6. Schafeer, JS., et al. 2009. J. Leukoc Biol. PubMed.
Description:
The isotype of RTK4530 immunoglobulin is rat IgG2b,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
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