The immunoglobulin was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The immunoglobulin solution should be stored undiluted at 4 °C
Application:
FC - Quality Tested IP*, WB*, ICC*, IF* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes:
The MG3-35 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofleorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 401310) as negative control.
Description:
The isotype of MG3-35 immunoglobulin is mouse IgG3,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
