Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes:
The MOPC-173 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofleorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 400224) as negative control.
Application References:
1. Luckashenak, N. A., et al., 2006. J. Immunol. 177:5177. 2. Burman, A. C., et al. 2007. Blood 110:1064. 3. Goo, S.Y., et al., 2007. J. Biol. Chem. doi:10.1074/jbc.M701876200. 4. Podolin, PL., et al. 2008. J Immunol.. 180:7989. PubMed
Description:
The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is Mouse IgG2a,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
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