The immunoglobulin was purified by affinity chromatography.
Storage & Handling:
The immunoglobulin solution should be stored undiluted at 4°C
Application:
FC - Quality Tested WB*, IP*, ICC*, IF*, IHC * - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes:
The MOPC-21 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofleorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 400124) as negative control.
Application References:
1. Carlsten,M.,et al.2007.Cancer Res.67:1317.PubMed 2. Smed-Sörensen, A., et al., 2008. Blood 111:5037. PubMed (FA)
Description:
The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
