The immunoglobulin was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The immunoglobulin solution should be stored undiluted at 4°C
Application:
FC, ICFC - Quality tested. WB*, IP*, ICC*, IF*, IHC* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes:
The MG1-45 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofleorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 401404) as negative control.
Application References:
1.Ozeki M.et al.2008. J Leukoc Biol.PubMed 2. Kraetzel, K., et al. 2008. Eur Respair J.PubMed 3. Ozeki, M., et al. 2008. J Leukoc Biol. PubMed 4. de Jong, MA., et al., 2008. J Gen Virol. 89:2398. PubMed
Description:
The isotype of MG1-45 immunoglobulin is mouse IgG1,κ. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
