The antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C.
Application:
FC, ICFC - Quality tested. WB*, IP*, ICC*, IF*, IHC* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
Application Notes:
The HTK888 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofleorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 400916) as negative control.
Application References:
1. Lesley, R., et al., 2006. PNAS 103:10717. 2. Yu, R., et al. 2006 Obesity 14:1353. 3. Yang, J.H.,et al.2005.Rheumatology(Oxford). 44:1245. PubMed 3. Mina-Osorio, P., et al. 2008. J. Leukoc Biol.PubMed 4. Shen, H., et al. 2009. J. Am Soc Nephrol. 20:1032. PubMed
Description:
The isotype of HTK888 immunoglobulin is Armenian hamster IgG. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
