Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Hou, J., 2005. Leuk Res 29:1059. PubMed 2. Thuerauf, DJ., et al. 2006. Circ Res.99:275. (WB) PubMed 3. Kim, KM., et al., 2007. Circ Res. 101:919. (WB) PubMed. 4. Zhou, X., et al., 2007. Leuk Res. 31:1259 PubMed. 5. Jiang, H., et al., 2007. Int J Hematol. 86:429 PubMed. 6. Romero-Ramirez, L., 2009. Transl Oncol. 2:31 PubMed. 7. Pattison, JS., et al., 2008. Circulation. 117:2743. (WB) PubMed. 8. Hsiao, JR., et al., 2009 Cancer Res 69:4461 (ChIP) PubMed 9. Guan, L., et al., 2009., Apoptosis. 14:218. PubMed 10. Scriven, P., et al., 2009. Br J Cancer. 101:1692 PubMed 11. Yu, J., et al ., 2009. Int J Biochem Cell Biol. 41:1739. PubMed
HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Xbp-1 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody recognizes the 54 kD splice variant of Xbp-1.
Description:
Xbp-1 (X-box binding protein 1) is a ubiquitously expressed 29 kD member of the CREB/ATF transcription factor family, and a mammalian homologue of the yeast transcription factor Hac1. Xbp-1 regulates MHC class II genes by binding to the X-box promoter motif and can also bind to the enhancer in T cell leukemia virus type I promoter to increase viral protein expression by binding the viral transactivator. This transcription factor is essential for hepatocyte differentiation and can be upregulated by ER stress, IL-6, and IL-4. The PAX5 transcription factor downregulates Xbp-1; downregulation has been shown to correlate with tumor progression in prostate cancer. The Poly6195 antibody recognizes the C-terminal region of human and rat Xbp-1 and has been shown to be useful for Western blotting.
Other Names:
X-box binding protein 1, XBP1
Structure:
Member of the CREB/ATF transcription factor family, mammalian homologue of the yeast transcription factor Hac1. Predicted molecular weight approximately 30 kD and 54 kD (splicing variant)
Distribution:
Ubiquitously expressed
Function:
Transcription factor regulating MHC class II genes by binding to X-box promoter motif. Can also bind to enhancer in T cell leukemia virus type I promoter and enhance viral protein expression by acting as DNA binding partner of a viral transactivator.
Regulation:
Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor
Antigen References:
1. Liou, H. C., et al., 1990. Science 247:1581. 2. Ponath, P. D., et al., 1993. J. Biol. Chem. 268:17074. 3. Takahashi, S., et al., 2002. Prostate 50:154.
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