Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Hou J. 2005. Leuk Res 29:1059. PubMed 2. Thuerauf DJ, et al. 2006. Circ Res.99:275. (WB) PubMed 3. Kim KM, et al. 2007. Circ Res. 101:919. (WB) PubMed 4. Zhou X, et al. 2007. Leuk Res. 31:1259 PubMed 5. Jiang H, et al. 2007. Int J Hematol. 86:429 PubMed 6. Romero-Ramirez L. 2009. Transl Oncol. 2:31 PubMed 7. Pattison JS, et al. 2008. Circulation. 117:2743. (WB) PubMed 8. Hsiao JR, et al. 2009 Cancer Res 69:4461 (ChIP) PubMed 9. Guan L, et al. 2009, Apoptosis. 14:218. PubMed 10. Scriven P, et al. 2009. Br J Cancer. 101:1692 PubMed 11. Yu J, et al. 2009. Int J Biochem Cell Biol. 41:1739. PubMed
Extracts of untreated HepG2 cells (lane 1) and extracts of HepG2 cells treated with 300 nM of thapsigargin for 16 hours (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified polyclonal anti-Xbp-1 antibody (clone Poly6195). The proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
Xbp-1 (X-box binding protein 1) is a ubiquitously expressed 29 kD member of the CREB/ATF transcription factor family, and a mammalian homologue of the yeast transcription factor Hac1. Xbp-1 regulates MHC class II genes by binding to the X-box promoter motif and can also bind to the enhancer in T cell leukemia virus type I promoter to increase viral protein expression by binding the viral transactivator. This transcription factor is essential for hepatocyte differentiation and can be upregulated by ER stress, IL-6, and IL-4. The PAX5 transcription factor downregulates Xbp-1; downregulation has been shown to correlate with tumor progression in prostate cancer. The Poly6195 antibody recognizes the C-terminal region of human and rat Xbp-1 and has been shown to be useful for Western blotting.
Other Names:
X-box binding protein 1, XBP1
Structure:
Member of the CREB/ATF transcription factor family, mammalian homologue of the yeast transcription factor Hac1. Predicted molecular weight approximately 30 kD and 54 kD (splicing variant)
Distribution:
Ubiquitously expressed
Function:
Transcription factor regulating MHC class II genes by binding to X-box promoter motif. Can also bind to enhancer in T cell leukemia virus type I promoter and enhance viral protein expression by acting as DNA binding partner of a viral transactivator.
Regulation:
Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor
Antigen References:
1. Liou HC, et al. 1990. Science 247:1581. 2. Ponath PD, et al. 1993. J. Biol. Chem. 268:17074. 3. Takahashi S, et al. 2002. Prostate 50:154.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Xbp-1 (COOH terminus)
Extracts of untreated HepG2 cells (lane 1) and extracts of HepG2 cells treated with 300 nM of thapsigargin for 16 hours (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified polyclonal anti-Xbp-1 antibody (clone Poly6195). The proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
