Human, Rat, and weak reactive with Mouse; recognizes the 54 kD form
Immunogen:
Peptide
Formulation:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20° C.
Application:
WB-Quality Tested IHC1 * - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Tsang, K. Y., et al., 2007. PLOS Biol. 5:568. 2. Thuerauf, DJ.,et al.2006. Circ Res.99:275.PubMed 3. Kim, KM.,et al.2007.Circ Res.101:919.PubMed 4. Pattison, JS.,et al. 2008. Circulation. 117:2743. PubMed
HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Xbp-1 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody recognizes the 54 kD splice variant of Xbp-1.
Description:
Xbp-1 (X-box binding protein 1) is a ubiquitously expressed 29 kD member of the CREB/ATF transcription factor family, and a mammalian homologue of the yeast transcription factor Hac1. Xbp-1 regulates MHC class II genes by binding to the X-box promoter motif and can also bind to the enhancer in T cell leukemia virus type I promoter to increase viral protein expression by binding the viral transactivator. This transcription factor is essential for hepatocyte differentiation and can be upregulated by ER stress, IL-6, and IL-4. The PAX5 transcription factor downregulates Xbp-1; downregulation has been shown to correlate with tumor progression in prostate cancer. The Poly6195 antibody recognizes the C-terminal region of human and rat Xbp-1 and has been shown to be useful for Western blotting.
Other Names:
X-box binding protein 1, XBP1
Structure:
Member of the CREB/ATF transcription factor family, mammalian homologue of the yeast transcription factor Hac1. Predicted molecular weight approximately 30 kD and 54 kD (splicing variant)
Distribution:
Ubiquitously expressed
Function:
Transcription factor regulating MHC class II genes by binding to X-box promoter motif. Can also bind to enhancer in T cell leukemia virus type I promoter and enhance viral protein expression by acting as DNA binding partner of a viral transactivator. Ess
Regulation:
Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor
Antigen References:
1. Liou, H. C., et al., 1990. Science 247:1581. 2. Ponath, P. D., et al., 1993. J. Biol. Chem. 268:17074. 3. Takahashi, S., et al., 2002. Prostate 50:154.
