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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The purified 14C11 antibody has been shown to be useful for Western blotting of human, mouse, and rat γ-tubulin.
Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against g-Tubulin (clone 14C11) followed by Rhodamine Red-X conjugated donkey anti-mouse IgG and DAPI.
Cell extract from A431 cells (Lane 1) or NIH3T3 cells (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-?-tubulin (Clone 14C11) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
γ-tubulin is a 51 kD member of the tubulin superfamily and is conserved between yeast and mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to be involved in cytoskeletal organization and mitosis. γ-tubulin is expressed at highest levels during prophase to metaphase and can be modified by phosphorylation. This protein has been shown to interact with α-tubulin, paxillin, and RAC GTPase activated protein 1.
Other Names:
γ-tubulin
Structure:
Tubulin superfamily, highly conserved between yeast and mammals. Predicted molecular weight approximately 51 kD
Distribution:
Cytoskeletal protein, high expressed in centrosomes
Function:
Cytoskeletal organization, mitosis
Regulation:
Expressed at highest levels during prophase to metaphase
Modification:
Phosphorylation
Interaction:
Interacts with α-tubulin, paxillin, RAC GTPase activated protein 1
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Tubulin-γ
Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against g-Tubulin (clone 14C11) followed by Rhodamine Red-X conjugated donkey anti-mouse IgG and DAPI.
Cell extract from A431 cells (Lane 1) or NIH3T3 cells (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-?-tubulin (Clone 14C11) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
